人工真皮成纤维细胞的分离培养与鉴定

目的:建立人工真皮成纤维细胞分离培养及鉴定方法.方法:取出生24 h内的Wistar大白鼠背部皮肤,分别用组织块法及酶消化法分离纯化、传代培养,用免疫组织化学方法进行鉴定.结果:用组织块法和酶消化法培养的成纤维细胞呈梭形,属纤维型细胞,免疫组织化学染色95%以上细胞中间丝波形蛋白呈棕色颗粒或棕色着染(阳性);结蛋白抗体和第Ⅷ因子相关抗原均无棕色颗粒或棕色着染(阴性),表明所培养的细胞为比较纯净的成纤维细胞.结论:该培养方法可提供较纯净的足够量的成纤维细胞.

Isolation,culture and identification of fibroblasts in artificial dermis

Objective: To explore the methods of isolation, culture and identification of fibroblasts in artificial dermis. Methods:The techniques of histotomy,degistation with enzymes and immunohistochemicstry were applied in the skin on the back of neonatal Wistar white rats. Results: With morphological analysitic methods the fusiform cells were fibroblasts and vimentin became brown granulations, immunohistochemic stain was positive and antigens found in 95% of those cells,but both antibodies against desmin eighth related with was negative. Conclusion: These techniques may provide us much purified and enough fibroblasts.