脑活素对液压冲击伤后原代神经细胞内游离钙的影响

目的探讨脑活素对液压冲击伤后神经细胞内游离钙的作用。方法原代培养的神经细胞分(1)正常组,(2)创伤组:液压冲击致神经细胞创伤后24h和48h组,(3)脑活素治疗组:分别于神经细胞创伤后0、1h给予1.25ml.L-1脑活素治疗(0h给药和1h给药组)。用激光扫描共聚焦显微镜和Ca2+荧光探针Fluo-3/AM观察细胞创伤后24h和48h细胞内游离钙[Ca2+]i的变化。结果神经元细胞创伤后,伤后24h神经元胞质内钙离子浓度达高峰,48h仍维持在较高水平;脑活素0或1h治疗后,在24h均能明显减轻创伤神经元细胞内的钙超载,48h后只有1h给予脑活素的细胞钙离子强度降低。结论脑活素对创伤的神经细胞有保护作用,且具有治疗时间窗。

Effects of cerebrolysin on intracellular calcium in primary cultured neurons after fluid percussion injury

Aim To investigate the changes of intracellular calcium in primary cultured neurons after fluid percussion injury under the effects of cerebrolysin.Methods The neurons of rats were divided into: normal group,FPI group and cerebrolysin group(0 h and 1 h treatment after fluid percussion injury(FPI)).The intracellular calcium([Ca2+]i) at rats neurons in 24 h and 48 h postinjury were measured by using the laser scanning confocal microscope under calcium fluorescent indicator Fluo-3/AM.Results The [Ca2+]i at rats neurons were markedly increased after 24 h postinjury compared with normal neurons and maintained the higher level after 48 h.Cerebrolysin,whenever added at 0h or 1h after FBI,could significantly decrease the rise of [Ca2+]i on 24 h postinjury,which only happened in 48 h postinjury by 1 h treatment after FPI.Conclusion Cerebrolysin has the protective effects on primary cultured rat cortical neurons of rats and has the time-window treatment.