米诺环素对大鼠局灶性脑缺血再灌注后ICAM-1表达的影响

目的观察米诺环素对大鼠短暂性脑缺血再灌注后细胞间黏附分子1表达的影响。方法采用改良线栓法制备大鼠大脑中动脉缺血2h再灌注24h模型,随机分为假手术组、缺血再灌注模型组、米诺环素处理组和米诺环素预处理组,每组16只。模型成功后观测各组大鼠的神经行为变化、脑梗死体积以及HE染色计数缺血区中性粒细胞的浸润数目,应用免疫组化方法检测细胞间黏附分子-1的表达情况。结果米诺环素能明显改善大鼠局灶性脑缺血再灌注引起的神经行为障碍,减少脑梗死体积,减少脑缺血引起细胞间黏附分子1的表达,抑制中性粒细胞的浸润。结论米诺环素对大鼠局灶脑缺血再灌足损伤有保护作用,抑制黏附分子可能是一种机制。

Influence of Minocycline on expression of intercellular adhesion molecule-1 in rats after focal cerebral ischemia reperfusion

Objective To observe the influence of Minocycline on the expression of intercellular adhesion molecule-1 (ICAM-1) protein after focal cerebral ischemia reperfusion in rats. Methods Rat models were established with filament method after 2-hour middle cerebral artery ischemia and 24-hour reperfusion, and were divided into four groups randomly:sham-operated group, model group, treatment group and pretreatment group with 16 rats in each group. After the model establishments,the change of neurological impairment and the volume of cerebral infarction were evaluated.The infiltration of neutrophil in ischemia areas was counted by hematoxylin-eosin staining, and the expression of ICAM-1 protein was tested by immunohistochemical method. Results Minocycline could improve the neurological impairment, decreased the infarcted volume and reduced the expressions of ICAM-1, restrained the infiltration of neutrophil so as to relieve the inflammatory reaction and had neuroprotective effect after focal cerebral ischemia. Conclusion Minocycline plays an neuroprotective effect after focal cerebral ischemia reperfusion by reducing the expressions of ICAM-1.