Subchronic toxicity study of Coptidis Rhizoma in rats |
| |
Authors: | Yong-Hoon Lee Duyeol Kim Mi Ju Lee Myoung Jun Kim Ho-Song Jang Sun Hee Park Jung-Min Lee Hye-Yeong Lee Beom Seok Han Woo-Chan Son Ji Hyeon Seok Jong Kwon Lee Jayoung Jeong Jin Seok Kang Jong-Koo Kang |
| |
Institution: | 1. Department of Pathology, Biotoxtech Co., Ltd, 686-2 Yangcheong-ri, Ochang-eup, Cheongwon-gun 363-883, Chungbuk, Republic of Korea;2. Division of Toxicopathology, Hoseo Toxicology Research Center, Hoseo University, 79 Hoseo-ro, BaeBang-Myeon, Asan 336-795, Chungnam, Republic of Korea;3. Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 138-736, Republic of Korea;4. Toxicological Research Divison, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osong, Cheongwon 363-700, Chungbuk, Republic of Korea;5. Department of Biomedical Laboratory Science, Namseoul University, 21 Maeju-ri, Seonghwan-eup, Cheonan 331-707, Chungnam, Republic of Korea;6. Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chungbuk National University, 410 Naesudong-ro, Heungdeok-gu, Cheongju 361-763, Chungbuk, Republic of Korea |
| |
Abstract: | Ethnopharmacological relevanceCoptidis Rhizoma (CR) is a medical herb from the family Ranunculacease that has been used to treat gastroenteritis, dysentery, diabetes mellitus, and severe skin diseases.Aim of the studyTo evaluate the no-observed-adverse-effect level (NOAEL) and the toxicity of CR, following repeat oral administration to rats for 13 weeks.Materials and methodsCR was administered by oral gavage to groups of rats (n=10/group, each sex) at dose levels of 0 (control), 25, 74, 222, 667 or 2000 mg/kg/day 5 times per week for 13 weeks. Mortality, clinical signs, body weights, food consumption, hematology, serum chemistry, urinalysis, vaginal cytology and sperm morphology, organ weights, gross and histopathological findings were compared between control and CR groups.ResultsUrinalysis showed a significant increase in N-acety1-β-glucosaminidase in males in the 2000 mg/kg/day group (P<0.01). However, no mortality or remarkable clinical signs were observed during this 13-week study. No adverse effects on body weight, food consumption, hematology, serum chemistry, organ weights, gross lesion, histopathology, vaginal cytology, sperm motility, or deformity were observed in the males or female rats treated with CR.ConclusionsOn the basis of these results, the NOAEL of CR is determined to be 667 mg/kg/day for males and 2000 mg/kg/day for females. |
| |
Keywords: | ANOVA analysis of variance CR Coptidis Rhizoma NOAEL no-observed-adverse-effect level NTP National Toxicology Program GLP good laboratory practice MFDS Ministry of Food and Drug Safety HPLC high performance liquid chromatography NAG N-acetyl-β-glucosaminidase CRE creatinine AST aspartate aminotransferase ALP alkaline phosphatase GGT gamma-glutamyl transpeptidase RBC total erythrocyte counts HGB hemoglobin concentration HCT hematocrit MCV mean cell volume MCH mean cell hemoglobin MCHC mean cell hemoglobin concentration PLT platelet count RBC total leukocyte counts NEU neutrophils EOS eosinophils Baso basophils Lym lymphocytes MONO monocytes RET reticulocytes PT prothrombin time APTT activated partial thromboplastin time ALT alanine aminotransferase AST aspartate aminotransferase ALP alkaline phosphatase SDH sorbitol dehydrogenase BUN blood urea nitrogen T-BIL total bilirubin TP total protein ALB albumin A/G albumin/globulin T-CHO total cholesterol TG triglycerides GLU glucose Ca calcium Cl chloride Na sodium K potassium |
本文献已被 ScienceDirect 等数据库收录! |
|