Confocal mirco-Raman spectroscopic analysis of the antioxidant protection mechanism of the oligosaccharides extracted from Morinda officinalis on human sperm DNA |
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Authors: | Di-Ling Chen Ning Li Li Lin He-ming Long Hui Lin Jun Chen He-Ming Zhang Chang-chun Zeng Song-Hao Liu |
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Institution: | 1. Southern Institute of Pharmaceutical Research, South China Normal University, Guangzhou, Guangdong 510631, People?s Republic of China;2. College of Chinese Materia Medical, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, People?s Republic of China;3. Department of Oncology, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, People?s Republic of China;4. Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510631, People?s Republic of China;5. School of Basic Medical Sciences, Guilin Medical University, Guilin, Guangxi 541004, People?s Republic of China |
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Abstract: | Ethnopharmacological relevenceMale infertility is a stressful and frustrating problem for the society, but a number of male infertility treatments are available as traditional Chinese medicine strategies which have been tried with variable success, while evidence is still limited on whether-or how much-herbs or supplements might help increase fertility, so the aim of this study was to investigate if the oligosaccharides extracted from Morinda officialis, a Chinese herb, is the active constituents to the fertility.Materials and methodsIn this study, we prepared the H2O2-demaged human sperm, cocultured with the oligosaccharides in vitro, then observed the changes of the DNA using confocal micro-Raman spectroscopy, and comparative analysis the differences of the spectra of different treated groups.ResultsThe results showed that the oligosaccharides extracted from Morinda officialis can keep the “Raman fingerprints” of the human sperm DNA almost the same as those of the control groups, but very different from the H2O2-induced groups, especially the intensity of bands at 787, 993, 1094, 1254, 1340, 1376, 1421, 1443, 1487, 1577 and 1662 cm−1 which could be as potential targets for the drugs finding, and further principal component analysis was successfully used to classify the Raman spectra of normal control and model groups.ConclusionThis results suggested that the oligosaccharides can protect the DNA of human sperm from being damaged by H2O2, and which was one of the active constituents of Morinda officialis on treating infertility. It was also demonstrated that Morinda officialis as a tonifying and replenishing natural herb medicine can be used to enhance reproductive functions, and the Raman spectroscopy could be an applicable technology for screening active components in vitro from herbs. |
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Keywords: | Hydrogen peroxide (PubChem CID:784) 1-kestose (PubChem CID:440080) Nystose (PubChem CID:166775) 1F-Fructofuranosylnystose (PubChem CID:3085157) Malondialdehyde (PubChem CID:10964) Phosphodiester (PubChem CID:16133915) Beta-2-desoxyribose (PubChem CID:642785) Alpha-2-desoxyribose (PubChem CID: 6427850) |
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