Effect of Ocimum basilicum L. on cyclo-oxygenase isoforms and prostaglandins involved in thrombosis |
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Authors: | Anwar Umar Wenting Zhou Elzira Abdusalam Arzigul Tursun Nadira Reyim Ibadet Tohti Nicholas Moore |
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Institution: | 1. Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China;2. Department of Pharmacology, University Bordeaux Segalen, 146 rue Leo Saignat, 33076 Bordeaux, France;3. Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang, PR China;4. Department of Cardiovascular Internal Medicine, The Second Affiliated Hospital of Xinjiang Medical University, 38 Nanhu Road, Shuimogou District, 830063 Urumqi, Xinjiang, PR China |
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Abstract: | Ethnopharmacological relevanceOcimum basilicum L. (OBL) is a plant used in traditional Uyghur medicine for the treatment and prevention of cardiovascular disease. In previous studies we had found an antihypertensive and antithrombotic effect suggestive of an effect on prostaglandins, which we attempt to document here.Materials and methods6-keto-PGF1α, the metabolite of prostacyclin, and PGE2 were measured in the supernatant of human umbilical vein endothelial cells (HUVEC) and basal or LPS-stimulated mouse coeliac macrophage cultures exposed to OBL ethanol (OBL-E) extracts and petroleum ether, chloroform, ethylacetate and butanol (PE, C, EA, B) fractions. In addition, 6-keto-PGF1α and thromboxane B2 (TXB2) were measured in a rat model of thromboangiitis obliterans exposed or not to OBL.ResultsShort-term exposure to OBL-E dose-dependently increased 6-keto-PGF1α from HUVEC, and long-term (24 h) exposure decreased it. OBL-C and OBL-B increased 6-keto-PGF1α, whereas the other fractions tended to decrease it after 24 h exposure. The extract and all fractions decreased basal and stimulated PGE2 production, but only OBL-EA and OBL-B reduced PGE2 in stimulated cultures to concentrations below the unstimulated values (P<0.05). In vivo OBL increased 6-keto-PGF1α and decreased TXB2.ConclusionOBL and its extracts increased 6-keto-PGF1α and reduced PGE2 and TXB2 production in a dose and time-related manner. This could indicate simultaneous inhibition of COX-2 and stimulation of endothelial COX-1. The butanol fraction seemed most promising in this respect. |
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Keywords: | 6-ketoPGF1α 6-keto-prostaglandin F1α AA arachidonic acid ADP adenosine diphosphate ANOVA analysis of variance ASA aspirin B butanol C chloroform CCTCC Wuhan University Cell Bank COX cyclo-oxygenase E ethanol EA ethylacetate FBS fetal bovine serum HUVEC human umbilical vein endothelial cells LPS lipopolysaccharide MCM mouse celiac macrophage OBL Ocimum basilicum L PE petroleum ether TXB2 thromboxane B2 |
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