Protective effect of Sheng-Nao-Kang decoction on focal cerebral ischemia-reperfusion injury in rats |
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Authors: | Lin Chen Ye Zhao Tianlong Zhang Xuan Dang Renming Xie Zhenzhi Li Yang Li Yuli Li Wenna Zhao Hongru Song |
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Institution: | 1. Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi''an 710069, PR China;2. College of Life Sciences, Northwest University, Xi''an 710069, PR China;3. The 323rd hospital of People''s Liberation Army, Xi''an 710069, PR China |
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Abstract: | Ethnopharmacological relevanceSheng-Nao-Kang decoction (SNK), a modified traditional Chinese medicine (TCM), has been used clinically for the treatment of acute and chronic cerebrovascular related diseases. To evaluate the protective effect of SNK on focal cerebral ischemia-reperfusion (I/R) injury in rats and investigate the underlying mechanisms.Materials and methodsFocal cerebral I/R injury in rats was induced by middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 24 h. Adult male Sprague-Dawley (SD) rats were randomly divided into six kinds of groups: Sham group; I/R group; SNK-treated groups at doses of 0.7 g/kg, 1.4 g/kg and 2.8 g/kg; and nimodipine (NMP)-treated group. The recoveries of neurological function in rats were estimated by neurological defect scoring and 2,3,5-triphenyltetrazolium chloride (TTC) staining after 24 h reperfusion. Various biochemical indexes in serum were assayed by colorimetry, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS) and total nitric oxide synthase (TNOS). Histological structures of the brain in rats were observed by hematoxylin and eosin (H&E) staining. Immunohistochemistry was performed to detect the caspase-3 protein content in rats.ResultsSNK administration significantly reduced the neurological defect scores and lessened the cerebral infarction volume. The treatment of SNK lowered MDA content, up-regulated SOD and GSH-Px levels, down-regulated iNOS and TNOS levels in serum. Furthermore, histological examination indicated that dense neuropil and largely surviving neurons were seen in SNK-treated rats. SNK administration restrained the expression of caspase-3 positive protein significantly.ConclusionThe results suggest that SNK demonstrates a strong and ameliorative effect on cerebral I/R damage in rats. The protective mechanisms of SNK are associated with its properties of anti-apoptosis and anti-oxidation as well as regulation of iNOS and TNOS. |
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Keywords: | ANOVA one-way analysis of variance CCA common carotid artery ECA external carotid artery GSH-Px glutathione peroxidase H& E hematoxylin and eosin HPLC high performance liquid chromatography ICA internal carotid artery iNOS inducible nitric oxide synthase IPP Image-Pro Plus I/R ischemia-reperfusion MCAO middle cerebral artery occlusion MDA malondialdehyde NMP nimodipine NO nitric oxide p o per os RNS reactive nitrogen radicals ROS reactive oxygen species SD Sprague-Dawley S E M standard error of mean SNK Sheng-Nao-Kang decoction SOD superoxide dismutase TCM traditional Chinese medicine TNOS total nitric oxide synthase TTC 2 3 5-triphenyltetrazolium chloride |
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