HLA-DQA1及DQB1等位基因与寻常型银屑病遗传易感性研究

目的 探讨HLA-DQA1和DQB1等位基因与汉族人寻常型银屑病遗传易感性。方法 利用聚合酶链反应-序列特异引物(PCR-SSP)法,对189例银屑病患者和273例健康人的HLA-DQA1和DQB1等位基因进行检测。结果 ①HLA-DQA1*0104和DQA1*0201与汉族人银屑病呈正相关性(Pc<0.05);DQA1*0501与汉族人银屑病呈负相关(Pc<0.001).②HLA-DQA1*0104、DQA1*0201和DQA1*0501等位基因与Ⅰ型银屑病发病有关。③HLA-DQA1*0104和DQA1*0201等位基因在有家族史和无家族史患者中的频率显着性增高。HLA-DQA1*0501仅在无家族史银屑病患者中显着性下降。结论 ①HLA-DQA1*0104和DQA1*0201可能是银屑病的易感基因或与易感基因相连锁;DQA1*0501等位基因可能具有阻止汉族人发生银屑病的作用。②有家族史和无家族史银屑病患者在其遗传背景上可能存在差异。

The Association of HLA- DQA1 and DQB1 Alleles with Genetic Susceptibility to Psoriasis Vulgaris in Han Chinese

Objective To explore HLA-DQA1 and DQB1 alleles in their association with genetic susceptibility to psoriasis vulgaris(PV) in Han Chinese. Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls. Results ①HLA-DQA1*0104 and DQA1*0201 alleles were positively associated with PV (Pc<0.05); HLA-DQA1*0501 allele was negatively associated with PV(Pc<0.001). ②HLA-DQA1*0104, DQA1*0201 and DAQ1*0501 alleles were associated with typeⅠpsoriasis only. ③HLA-DQA1*0104 and DQA1*0201 alleles were increased in patients with or without family history. DQA1*0501 allele was only decreased in patients without family history. Conclusions ①HLA-DQA1*0104 and DQA1*0201 alleles may be the susceptible genes or have close linkage with susceptible genes. HLA-DQA1*0501 allele may have protective effects on development of PV in the Chinese. ②There may be a difference in genetic background between psoriasis patients with and without family history.