狗牙髓和小肠碱性磷酸酶的电泳分离

作用正丁醇抽提法从狗牙髓和小肠粘膜制备碱性磷酸酶，并探讨了这两种组织来源的碱性磷酸酶的电泳分离方法。结果表明牙髓碱性磷酸酶和小肠碱性磷酸酶的酶染色带谱显不同，前呈２条带，后呈４条带。两种碱性磷酸酶制剂经神经氨酸酶处理后均表现为迁移率碱小。在电泳系统中加入０．５％Ｔｒｉ－ｔｏｎＸ－１００，可显提高酶染色带的分辨力。作还比较了圆盘聚丙烯酰胺凝胶电泳和平板琼脂糖凝胶电泳的效果，结果表明，圆盘

The electrophoretic separation of canine pulpal and small intestinal alkaline phosphatases

We have prepared alkaline phosphotases (ALP) from canine pulps and small intestines by n-bu-tanol extractions, and studied the electrophoretic resolution of these two tissue derived alkaline phosphotases. It was found that the pulpal and the intestinal alkaline phosphatases shows quite different enzymatic electrophoresis bands, the pulp ALP was shown in two bands and the intestine ALP in four bands. After being treated with neuraminidase, both kinds of ALP migrated slightly slower than the intact ALP. The resolutions of ALP were markedly improved when the electrophoresis buffer system contains 0. 5% Triton X-100. Furthermore, the polyacrylamide gel disc electrophoresis was compared with agarose gel horizontal slab electrophoresis. And it was revealed that the polyacrylamide gel system yielded better resolution than the agarose gel system. Yet the agarose gel system is easier to manipulate and to make comparative analysis.