| Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture |
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| 引用本文： | 张亚洁,许红霞,曾凯宏,糜漫天. Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture[J]. 中国人民解放军军医大学学报, 2007, 22(3): 137-142. DOI: 10.1016/S1000-1948(07)60030-6 |
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| 作者姓名： | 张亚洁 许红霞 曾凯宏 糜漫天 |
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| 作者单位： | Department of Nutrition and Food Hygiene Key Laboratory of Nutrition and Food Security of Chongqing，College of Preventive Medicine，Third Military Medical University，Department of Nutrition and Food Hygiene，Key Laboratory of Nutrition and Food Security of Chongqing，College of Preventive Medicine，Third Military Medical University，Department of Nutrition and Food Hygiene，Key Laboratory of Nutrition and Food Security of Chongqing，College of Preventive Medicine，Third Military Medical University，Department of Nutrition and Food Hygiene，Key Laboratory of Nutrition and Food Security of Chongqing，College of Preventive Medicine，Third Military Medical University，Chongqing 400038，China，Chongqing 400038，China，Chongqing 400038，China，Chongqing 400038，China |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No. 30571570) |
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| 摘 要： | Objective： To detect the expression of glial fibrillary acid protein （GFAP） and taurine transporter （TauT） in the retinal Müller cells in high glucose culture with taurine and to explore the influence of glucose on the taurine transporting, and the possible protective effects of taurine on MUller cells in early diabetic retinopathy. Methods： The Müller cells from the rat retina were cultured in high glucose, and GFAP and Taut expressions were detected in the cells treated with different doses of taurine by immuocytochemical fluorescein staining and Western blotting. Results： High glucose enhanced the expression of GFAP and decreased the expression of TauT in Müller cells. Taurine decreased the up-regulation of GFAP in the cells which was induced by high glucose; 0. 1-10 mmol/L taurine increased the expression of TauT in Müller cells. Conclusion： Taurine can inhibit the changes in Müller cell resulted from high glucose.
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| 关 键 词： | 小鼠 动物实验 神经胶质 免疫荧光反应 |
| 收稿时间： | 2007-03-30 |
Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture |
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| ZHANG Ya-jie,XU Hong-xia,ZENG Kai-hong,MI Man-tian,. Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture[J]. Journal of Medical Colleges of PLA(China), 2007, 22(3): 137-142. DOI: 10.1016/S1000-1948(07)60030-6 |
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| Authors: | ZHANG Ya-jie XU Hong-xia ZENG Kai-hong MI Man-tian |
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| Affiliation: | [1]Department of Nutrition and Food Hygiene, Key Laboratory of Nutrition and Food Security of Chongqing, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | ObjectiveTo detect the expression of glial fibrillary acid protein (GFAP) and taurine transporter (TauT) in the retinal Müller cells in high glucose culture with taurine and to explore the influence of glucose on the taurine transporting, and the possible protective effects of taurine on Müller cells in early diabetic retinopathy.MethodsThe Müller cells from the rat retina were cultured in high glucose, and GFAP and TauT expressions were detected in the cells treated with different doses of taurine by immuocy-tochemical fluorescein staining and Western blotting.ResultsHigh glucose enhanced the expression of GFAP and decreased the expression of TauT in Müller cells. Taurine decreased the up-regulation of GFAP in the cells which was induced by high glucose; 0. 1–10 mmol/L taurine increased the expression of TauT in Müller cells.ConclusionTaurine can inhibit the changes in Muller cell resulted from high glucose. |
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| Keywords: | Müller cells taurine taurine transporter glial fibrillary acid protein immunocytochemical fluorescein staining |
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