双波长HPLC法同时测定鼻渊宁颗粒中栀子苷和靛玉红的含量

目的:建立同时测定鼻渊宁颗粒中栀子苷和靛玉红含量的方法。方法:采用双波长高效液相色谱法。色谱柱为Shim-pack VP-ODS C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%醋酸溶液(梯度洗脱),流速为0.8 ml/min,柱温为30℃,检测波长为238、289 nm。结果:栀子苷和靛玉红的质量浓度分别在3.5⁵2.5、2.052.5、2.0⁴0.0 mg/L范围内与各自峰面积积分值呈良好线性关系(r均为0.999 9);精密度、稳定性、重复性试验的RSD<2.69%;平均加样回收率分别为98.9%和99.9%,RSD分别为1.2%和0.6%(n均为6)。结论:该方法简便、快速、专属性强,结果准确、可靠,可用于鼻渊宁颗粒的质量控制。

Simultaneous Determination of Geniposide and Indirubin in Biyuanning Granules by Dual-wavelength HPLC

OBJECTIVE： To establish a method for the content determination of geniposide and indirubin in Biyuanning granules. METHODS： Dual-wavelength HPLC was adopted. The determination was performed on Shim-pack VP-ODS C18（250 mm×4.6 mm, 5μm） column with mobile phase consisted of acetonitrile-0.5% acetic acid （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelengths were set at 238 and 289 nm. RESULTS： The linear ranges were 3.5-52.5 mg/L for geniposide and 2.0-40.0 mg/L for indirubin （r=0.999 9）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.69%. The average recoveries were 98.9% and 99,9% （n=6）, respectively. CONCLUSIONS： The method is simple, accurate and reproducible, which can provide reference for quality evaluation of Biyuanning granules.