| 蜈蚣败毒饮对HaCaT细胞Keratin6 mRNA表达的影响 |
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| 引用本文： | 杨素清,;魏巍,;闫景东,;安月鹏.蜈蚣败毒饮对HaCaT细胞Keratin6 mRNA表达的影响[J].中国药房,2014(47):4420-4422. |
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| 作者姓名： | 杨素清 ;魏巍 ;闫景东 ;安月鹏 |
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| 作者单位： | [1]黑龙江中医药大学附属第一医院皮肤科,哈尔滨150040; [2]黑龙江中医药大学临床医学院,哈尔滨150040 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目（No.12531619） |
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| 摘 要： | 目的:研究蜈蚣败毒饮对角质形成(Ha Ca T)细胞角蛋白Keratin6 m RNA表达的影响。方法:36只Wistar大鼠随机均分为空白对照(等容生理盐水)组、甲氨蝶呤(0.22 g/100 g)组、复方青黛胶囊(0.26 g/100 g)组与蜈蚣败毒饮高、中、低剂量(11.88、5.94、2.96 g/100 g)组,ig给药,每天2次,连续3 d。末次给药后取腹主动脉血,各组血清作用于相应的Ha Ca T细胞培养24 h。通过实时荧光酶链聚合反应(RT-PCR)法测定Ha Ca T细胞Keratin6 m RNA的表达。结果:与空白对照组比较,各用药组Ha Ca T细胞Keratin6 m RNA表达减弱,蜈蚣败毒饮在减弱Ha Ca T细胞Keratin6 m RNA表达方面呈现出非剂量依赖关系。结论:蜈蚣败毒饮可能通过抑制Keratin6 m RNA的表达,从而降低了Ha Ca T细胞增殖来发挥抗银屑病作用。
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| 关 键 词： | 蜈蚣败毒饮 银屑病 角质形成细胞 角蛋白 信使RNA |
Effects of Wugong Baiduyin on mRNA Expression of Keratin6 in HaCaT Cells |
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| Institution: | YANG Su-qing, WEI Wei, YAN Jing-dong, AN Yue-peng ( 1.Dept. of Dermatology, The First Affiliated Hospital of Heilongjiang University of TCM, Harbin 150040, China; 2.College of Clinical Medicine, Heilongjiang University of TCM, Harbin 150040, China) |
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| Abstract: | OBJECTIVE： To study the effects of Wugong baiduyin on mRNA expression of Keratin6 in HaCaT cells. METHODS： 36 Wistar rats were randomly divided into blank control group （constant volume of normal saline）, methotrexate group （0.22 g/100 g）, Compound qingdai capsule group （0.26 g/100 g） and Wugong baiduyin high-dose, medium-dose and low-dose groups （ 11.88, 5.94, 2.96 g/100 g）, They were given relevant medicine intragastrieally twice a day for consecutive 3 d. Blood samples were collected from aorta abdominalis after last administration, and HaCaT cells were cultured for 24 h. mRNA expression of Keratin6 in HaCaT cells was determined by RT-PCR. RESULTS： Compared with blank control group, mRNA expression of Keratin6 in HaCaT cells were decreased in other groups, and Wugong baiduyin decreased mRNA expression of Keratin6 in HaCaT cells in nondose dependent. CONCLUSIONS： Wugong baiduyin plays the role of anti-psoriasis through inhibiting the mRNA expression of Keratin6 and decreasing the proliferation of HaCaT cells. |
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| Keywords: | Wugong baiduyin Psoriasis HaCaT Cell Keratin mRNA |
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