HPLC法同时测定舒肝解郁胶囊中金丝桃苷和芦丁的含量

目的:建立同时测定舒肝解郁胶囊中金丝桃苷、芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm),流动相为乙腈-甲醇(90∶10,V/V)-50 mmol/L磷酸盐缓冲液(梯度洗脱),流速为0.8 ml/min,检测波长为360 nm,柱温为室温。结果:金丝桃苷和芦丁的进样量分别在1.272¹27.240、1.393127.240、1.393¹39.350μg范围内与峰面积积分值呈良好线性关系(r=0.999 9);二者精密度、稳定性、重复性的RSD<2%;平均加样回收率分别为96.9%、98.6%(n均为9)。结论:该方法快速、准确,重复性好,可用于舒肝解郁胶囊的质量控制。

Simultaneous Determination of Rutin and Hyperoside in Shugan Jieyu Capsule by HPLC

OBJECTIVE： To develop a method for simultaneous determination of rutin and hyperoside in Shugan jieyu capsule. METHODS：HPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18（250 mm×4.6 mm, 5μm） column with mobile phase consisted of acetonitrile-methanol （90： 10, V/V）-50 mmol/L phosphate buffer （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelength was set at 360 nm, and the column temperature was room temperature. RESULTS： The linear ranges were 1.272-127.240μg for hyperoside and 1.393-139.350μg for rutin, respectively （r=0.999 9）. RSDs of precision, stability and reproducibility tests were all lower tharn 2%. The average recoveries were 96.9% and 98.6% （n=9）, respectively. CONCLUSIONS ： The method is rapid, accurate and repeatable, and can be used for quality control in Shugan jieyu capsule.