Biochemical Analysis and Characterization of Proteins in Mouse Embryo and Uterine Tissue at Peri-implantation Stage

The patterns of total protein synthesis by the mouse early embryos during the peri-implantation period and by the uterine tissue from the 2-cell to the blastocyst stages were analysed.There were large quantities of acid proteins in the developing embryos.Two specific proteins were found with PI5.5 in the blastocyst and PI9.0 morula and blastocyst.On the other hand,2 specific proteins with PI5.05 and 5.70 were found only in the pregnant uterine tissue and flushings,but not in non-pregnant samples. Based on the results of the present investigation,it is reasonable to assume that changes of protein synthesis both in the embryos and in the uterine tissue during the peri-implantation period may be required for the establishment of a successful and normal pregnancy.