| 金属离子对单核/巨噬细胞细胞活性及其膜上RANK表达的影响 |
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| 引用本文： | 戴闽,陈锐,詹平,袁晓军,艾江波,程明.金属离子对单核/巨噬细胞细胞活性及其膜上RANK表达的影响[J].中国组织工程研究与临床康复,2010,14(4). |
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| 作者姓名： | 戴闽 陈锐 詹平 袁晓军 艾江波 程明 |
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| 作者单位： | 南昌大学第一附属医院骨二科,江西省南昌市,330006 |
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| 基金项目： | 课题受国家自然科学基金资助 |
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| 摘 要： | 背景:与其他配伍的假体一样,金属一金属假体在使用过程中也会产生大量的磨损颗粒和金属离子,其中金属离子以钴和铬离子较为常见,可导致假体周围骨溶解,引起假体无菌性松动.目的:观察Co~(2+)、Cr~(3+)离子对体外培养小鼠单核,巨噬细胞(RAW264.7)的细胞活性及其膜上RANK表达的影响.方法:体外培养单核,巨噬细胞(RAW264.7),采用金属钴铬离子对单核,巨噬细胞进行干预,不同时间点用-四唑盐比色方法检测细胞活性并以半定量反转录一聚合酶链反应方法测定RANK mRNA的表达量.结果与结论:四唑盐比色实验结果显示,与对照组相比,Co~(2+)、Cr~(3+)可使单核,巨噬细胞的细胞活性明显下降.单核/巨噬细胞暴露在钴铬离子下,与对照组相比,钴铬离子组单核,巨噬细胞RANKmRNA在12 h表达增强(P<0.05),24 h达到高峰(P< 0.05),48 h较24 h表达下降(P<0.05).结果提示,金属离子对单核/巨噬细胞有细胞毒性,且能够刺激单核/巨噬细胞RANK mRNA的表达,为单核/巨噬细胞向具有骨质吸收功能的破骨样细胞转化提供必要的前提条件.
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| 关 键 词： | 钴离子 铬离子 单核/巨噬细胞 人工假体 |
Effect of metal ions on monocyte-macrophage cells viability and RANK expression |
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| Dai Min,Chen Rui,Zhan Ping,Yuan Xiao-jun,Ai Jiang-bo,Cheng Ming.Effect of metal ions on monocyte-macrophage cells viability and RANK expression[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(4). |
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| Authors: | Dai Min Chen Rui Zhan Ping Yuan Xiao-jun Ai Jiang-bo Cheng Ming |
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| Institution: | Dai Min,Chen Rui,Zhan Ping,Yuan Xiao-jun,Ai Jiang-bo,Cheng Ming Second Department of Orthopaedics,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China |
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| Abstract: | BACKGROUND: Similar to other prosthesis, metal-metal prosthesis would produce plenty of wear particles and metal ions, mainly presented as cobalt (Co2+) and chromium (Cr3+), which can lead to osteolysis, eventually, result in aseptic loosening. OBJECTIVE: To observe the effect of Co2+ and Cr3+ ions on the cells viability and expression of RANK in rats monocyte-macrophage cells (RAW264.7) in vitro. METHODS: Monocyte-macrophage cells (RAW264.7) were cultured in vitro, and then the cells were exposed to Co2+ a... |
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| Keywords: | RANK |
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