组织细胞性坏死性淋巴结炎

目的：总结10例组织细胞性坏死性淋巴结炎（HNL）的临床病理特征。方法：复查10例淋巴结活检标本的HE切片；分别用S－P法和TUNEL法检测细胞免疫表型和凋亡。结果：5例曾被诊断为非霍奇金淋巴瘤。组织学上副皮质区，尤其滤泡间区有各种大小散在或融合病灶，由多种形态的组织细胞、转化淋巴细胞以及凋亡碎片组成，缺乏粒细胞浸润。淋巴细胞表达CD3和CD45RO，不表达CD15、CD20、CD30；组织细胞表达CD68和（或）Mac387。结论：淋巴结活检显示有多种组织细胞、转化T细胞以及凋亡碎片组成的病灶，不合并有粒细胞浸润，有利于HNL的诊断。

Histiocytic necrotizing lymphadenitis

Purpose To describe clinicopathological and immunophenotypic features of 10 cases of histiocytic necrotizing lymphadenitis (HNL). Methods HE sections of 11 lymph node biopsies were re examined. Immunophenotyping and detection of apoptotic DNA fragments were performed using S P and TUNEL methods, respectively. Results Five cases have been diagnosed as non Hodgkin lymphoma. Histologically variable sized discrete or confluent nodules were seen in the paracortex, especially in the interfollicular area, which were composed of proliferative pleomorphic histiocytes, transformed lymphocytes, and karyorrhectic debris. Immunohistochemistry revealed CD3+ and CD45RO+ for lymphocytes, Mac387+ and/or CD68+ for histiocytes, and no expression for CD15,CD30 and CD20 in the lesions. Conclusions The presence of pleomorphic histiocytes, transformed T cells, and karyorrhectic debris in the biopsy of lymph nodes, together with the absence of neutrophils support the diagnosis of HNL.