| 大鼠运动区脑片培养及皮层锥体细胞的组化鉴定 |
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| 引用本文： | 肖向建,刘卫刚,马征,李敏,李春岩. 大鼠运动区脑片培养及皮层锥体细胞的组化鉴定[J]. 脑与神经疾病杂志, 2005, 13(1): 9-11 |
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| 作者姓名： | 肖向建 刘卫刚 马征 李敏 李春岩 |
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| 作者单位： | 1. 河北省人民医院康复科
2. 河北医大第二医院神经内科 |
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| 基金项目： | 河北省自然科学基金资助项目(编号 303487) |
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| 摘 要： | 目的:探讨大鼠运动区脑片器官型培养的方法及其皮层第V层锥体细胞的鉴定。方法:利用出生后1天的乳鼠大脑活组织切片建立脑片的器官型培养模型,并用抗非磷酸化神经丝单克隆抗体SM-32对皮层锥体细胞加以鉴定。结果:脑片体外生长良好,形态完整,皮层锥体细胞数目恒定,并可存活1个月以上。结论:大鼠脑片的器官培养技术为研究大脑生理、病理改变及神经保护提供了有效的方法。
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| 关 键 词： | 脑片 器官型培养 锥体细胞 非磷酸化神经丝 |
| 文章编号： | 1006-351X(2005)01-0009-03 |
Rat brain motor area slice organotypic culture and the identification of pyramidal cell |
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| Affiliation: | ( Department of Neurology,the Second Hospital,HeBei Medical University,Shijia zhuang,Hebei province 050000) |
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| Abstract: | Objective: : To establish a method of brain slice organotypic culture for neonate rats and provide a method of identification of pyramidal cells m layer V. Methods. Organotypic brain slice cultures were prepared using brain from 1-day-old rat pups. Pyramidal cells' survival was evaluated by culture morphology and nonphosphorylated neurofilament H (SMI-32) immunohistochemistry staining. Results: The brain explants can be maintained in culture for more than 1 month with excellent organotypic cellular organization and a stable population of cortical pyramidal cells in layer V. Conclusion: Organotypic cultures provide an effective method to study the physiological, pathological changes and neuroprotection of brain. |
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| Keywords: | brain slice organotypic culture pyramidal cell nonphosphorylated neurofilament H(SMI-32) |
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