| 胚胎神经上皮干细胞体外克隆增殖与分化的实验研究 |
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| 引用本文： | 王家增,薛良华,赵遵杭,孙晋浩.胚胎神经上皮干细胞体外克隆增殖与分化的实验研究[J].山东医学高等专科学校学报,2008,30(3). |
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| 作者姓名： | 王家增 薛良华 赵遵杭 孙晋浩 |
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| 作者单位： | 1. 山东医学高等专科学校,山东临沂,276002
2. 山东大学医学院人体解剖学教研,室,山东济南,250012 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 目的观察胚胎神经上皮干细胞在体外克隆增殖和诱导分化的情况。方法取孕11 d大鼠的胚胎,经分离获得神经管上皮细胞,在无血清培养基内克隆、增殖、传代。取不同时期的神经球进行神经干细胞的鉴定和诱导分化,并通过免疫组织化学染色鉴定分化的结果。结果胚胎神经上皮干细胞在体外能够克隆、增殖,形成的神经球呈nestin阳性,在分化培养基内可分化为星型胶质细胞和神经元。结论胚胎神经上皮干细胞可在体外进行单细胞克隆和诱导分化。
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| 关 键 词： | 胚胎 神经上皮 干细胞 增殖 分化 |
THE AMPLIFICATION AND DIFFERENTIATION OF THE EMBRYONIC NEUROEPITHELIAL STEM CELLS IN VITRO |
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| WANG Jia-zeng,XUE Liang-hua,ZHAO Zun-hang,et al.THE AMPLIFICATION AND DIFFERENTIATION OF THE EMBRYONIC NEUROEPITHELIAL STEM CELLS IN VITRO[J].Journal of Shandong Medical College,2008,30(3). |
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| Authors: | WANG Jia-zeng XUE Liang-hua ZHAO Zun-hang |
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| Abstract: | Objective To observe the amplification and differentiation of the embryonic neuroepithelial stem cells in vitro.Methods The neuroepithelial stem cells were obtained from the E11d embryos,and cultured in the free-serum medium.These cells were identified and induced during different culturing periods in vitro by immunocytochemistry.Results The embryonic neuroepithelial stem cells could amplify through cloning and the neurospheres were nestin-positive.The neurons and astrocytes were observed in the differentiation medium.Conclusion The embryonic neuroepithelial stem cells can grow and amplify by single cloning and differention by inducing in vitro. |
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| Keywords: | Neuroepithelium Stem cells Embryo Amplification Differentiation |
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