改进TRAP-PCR检测端粒酶活性对早期胃癌的诊断

目的探索端粒酶活性早期诊断恶性实体瘤的新方法．方法通过胃癌组织的分离和肿瘤细胞系的培养，用改良ＴＲＡＰＰＣＲ方法，来检测其端粒酶的活性．结果胃癌组织及肿瘤细胞系的端粒酶总表达率９３％（２５／２７），其中细胞系ＭＫＮ２８，ＭＫＮ４５，ＨＨＣＬ，ＳＧＣ７９０１及Ｔｃａ８１１３均为阳性表达；胃癌远隔的正常粘膜标本表达率为０％（０／２０）；其中有２００个肿瘤细胞存在便可测得其端粒酶的活性．结论胃癌组织及肿瘤细胞系的端粒酶表达，具有很强的肿瘤特异性和较高的表达率；改进ＴＲＡＰＰＣＲ方法具有提高恶性实体瘤早期诊断的潜在价值

Modified TRAP PCR in detection of telomerase activity in early diagnosis of stomach tumors *

AIM To study modified TRAP PCR in detection of telomerase activity in early diagnosis of stomach tumors. METHODS By the modified method of TRAP PCR, telomerase activity was assessed in cultures of tumor cell lines as well as in tripsized gastric cancer tissue. RESULTS The overall rate of telomerase expression was 93% (25/27) in tumor tissues,and no expression was observed in samples of normal mucosa (0/20). Telomerase expression was present in all MKN28, MKN45, HHCL, SGC7901 and Tca8113 tumor cell lines. Telomerase activity was detectable when the number of tumor cells exceeded 200. CONCLUSION High telomerase expression is specific in all solid tumors so that its detection might bear great importance in the early diagnosis of various malignancies.