应用血清蛋白组学技术筛选鼻咽癌肿瘤抗原

目的: 应用血清蛋白组学技术优化肿瘤抗原筛选方法,筛选鼻咽癌肿瘤抗原.方法: 以免疫印迹技术筛选出含有特异性抗体的鼻咽癌患者血清,运用免疫沉淀技术对血清与鼻咽癌细胞株(CNE1)的裂解物进行处理以富集肿瘤抗原,正常人血清做对照,经凝胶电泳找出差异条带,通过质谱分析、数据库搜索和信息学分析鉴定差异蛋白.结果: 应用此方法找出存在于鼻咽癌样本中的明显差异条带1条,重复性较好,经鉴定该条带为膜结合IgM.结论: 采用上述方法筛选肿瘤抗原行之有效,可能成为肿瘤抗原筛选的一种简单、便捷、灵敏的有效方法,得到鼻咽癌候选抗原膜结合IgM.

Screening Tumor Antigens of Nasopharyngeal Carcinoma by Serological Proteomics Technologies

Objective:To screen tumor antigens of nasopharyngeal carcinoma by serological proteomics technologies. Methods:The nasopharyngeal carcinoma cell line CNE-1 was used as a source of tumor cell proteins for Western blot a-nalysis in which individual sera with special immunoreaction were selected and for immunoprecipitation analysis. Then, differential proteins are analyzed by mass spectrometry and bioinformatics methods. Results:Membrane-bound IgM was i-dentified in the differential protein band. Conclusion:It may be a convenient, simple and sensitive way to screen tumor antigens by proteomics technologies. Membrane-bound IgM may probably be a novel antigen of nasopharyngeal carcinoma.