氢气饱和生理盐水对百草枯中毒大鼠肺组织的作用

目的 探讨氢气饱和生理盐水对百草枯(PQ)中毒大鼠肺损伤及肺纤维化的保护作用.方法 该实验在南昌大学第一附属医院动物实验室完成.48只SD大鼠随机分为对照组、染毒组和干预组,每组16只.染毒组及干预组给予50 mg/kgPQ一次性灌胃染毒,对照组给予等量蒸馏水灌胃.干预组大鼠在染毒1 h后开始予腹腔注射氧气饱和生理盐水5 mL/kg,2次/d,直至处死;染毒组及对照组大鼠按体质量给予等量生理盐水腹腔内注射.于染毒第3天及第21天分别测定各组大鼠动脉血氧分压(PaO2)、肺组织8-羟基脱氧鸟苷(8-OHDG)含量及转化生长因子β1(TGF-31)的表达.结果 (1)染毒后3 d,与对照组比较,染毒组及干预组PaO2均明显下降(P＜0.01);与染毒组比较,干预组PaO2有所改善(P＜0.05).21 d后,与对照组比较,染毒组PaO2仍明显减低(P＜0.01),干预组有一定程度下降(P＜0.05);与染毒组比较,干预组PaO2明显升高(P＜0.01).(2)染毒后3 d,与对照组比较,染毒组8-OHDG含量明显增加(P＜0.01),干预组亦有一定程度增加(P＜0.05);与染毒组比较,干预组8-OHDG含量有明显下降(P＜0.01).21 d后,各组间比较差异无统计学意义(P＞0.05).(3)染毒后3 d,与对照组比较,染毒组TGF-β1表达明显增加(P＜0.01),干预组有一定程度增加(P＜0.05);干预组较染毒组间比有一定下降(P＜0.05).21 d后,与对照组比较,染毒组TGF-β1表达明显增加(P＜0.01),干预组有一定增加(P＜0.05);与染毒组比较,干预组TGF-β1表达明显下降(P＜0.01).结论 氢气饱和生理盐水能改善PQ大鼠氧化应激状态,减轻肺氧化损伤,降低肺纤维化程度.

Effects of hydrogen saturated saline on the lung after paraquat poisoning

Objective To investigate protective effects of the hydrogen saturated saline on acute lung injury and pulmonary fibrosis in rats with paraquat (PQ) poisoning. Method Forty-eight Sprague-Dawley rats were randomly divided into 3 groups, namely control group, PQ poisoning group and intervention group (n = 16 rats in each group) . Animals in PQ poisoning group and intervention group were fed with PQ in dosage of 50 mg / kg. Rats of control group were fed equivalent amount of distilled water instead. One hour after administration of PQ, rats of intervention group were treated with 5 ml / kg hydrogen saturated saline injected intra-peritoneally twice a day until the rats were sacrificed. The rats of poisoning group and control group were treated with intra-peritoneal injection of equivalent amount of normal saline. Arterial partial pressure of oxygen ( PaO2 ), 8-hydroxy-2' -desoxyguanosine (8-OHDG) and transforming growth factor β1 (TGF-β1) of lung tissue were measured on the 3rd and 21st day after PQ administration. Quantitative data was expressed as mean ±standard deviation (x-±s) . SPSS version 12. 0 package was applied for variance analysis and SNK-q test, and statistical differences were considered significant when P ＜ 0. 05. Results ① PaO2 decreased significantly in poisoning group (9. 34 ± 0. 47 kPa) and intervention group (10. 30±0.62 kPa) compared with control group (11.87 +/- 0.42 kPa) on the 3d (P ＜0.01), and as intervention group was compared with poisoning group, there was a significant difference ( P ＜ 0. 05 ) . On the 21st day, PaO2 was still lower in poisoning group (8. 36 ±0. 51 kPa) and intervention group ( 10. 14 ±0. 27 kPa) than that in control group ( 11.87 +0. 24 kPa) (P ＜0. 01 and P ＜0. 05, respectively), and as intervention group was compared with poisoning group, there was a significant difference ( P ＜ 0. 01 ) . ②The levels of 8-OHDG in lung tissue increased significantly in poisoning group (23.58±7. 18 ng/ml ) and intervention group (9. 49± 2. 45 ng/ml) on the 3rd day after PQ administration compared with control group (7.71 + 1.96 ng/ml) (P＜0. 01 and P＜0. 05, respectively), and as intervention group was compared with poisoning group there was a significant difference ( p ＜0. 01 ) . There were no significant differences in 8-OHDG level found among the groups on the 21st day after PQ administration (P ＞ 0. 05 ) . ③ The level of TGF-β1 (measured by mean optic density, MOD) in lung tissue of rats in poisoning group ( 10. 11±2.49 MOD) and intervention group (8. 14 + 1.58 MOD) exhibited in higher levels than control group (5.93 + 1. 98 MOD) on the 3rd and (5.97 + 2. 35 MOD) on the 21st day after PQ administration (P ＜0. 01 and P ＜0. 05, respectively), and however, a lower level of TGF-β1 was observed in intervention group on 3d and 21d compared with poisoning group (P ＜0. 05 and P ＜0. 01, respectively) . Conclusions Hydrogen saturated saline can alleviate oxidative stress, mitigate oxidative damage and inhibit pulmonary fibrosis of lung induced by PQ intoxication.