臂丛神经根性撕脱伤动物模型的研究

目的 研究臂丛神经根性撕脱伤实验动物模型。方法 选用Wistar大白鼠，取右下颌骨向下至胸大肌中部皮肤切口，显露胸大肌，分别切断胸大肌、胸小肌；暴露井剪断右锁骨，镜下解剖分离臂丛神经并紧贴近端根部将颈5至颈8剪断，造成臂丛神经根性撕脱伤模型，实验组用颈3、颈4神经修复臂丛中已损伤的颈5、颈6神经，对照组不做修复；用电生理及组织病理学观察神经肌肉组织变化，结果术后3个月病理检查发现，实验组中肌皮神经可见少量神经纤维长入，肱二共肌萎缩轻，失神经组之肌皮神经可见神经纤维变性、崩解、吸收，肱二头肌萎缩明显，电生理检查显示实验组与失神经组之诱发电位峰值电压与刺激电流比值及潜伏期时间与刺激电流强度之比值在统计学上有显著性差异（P〈0．05）。结论 该实验模型操作简单，科学，合理。为臂丛神经根性撕脱伤实验研究奠定了基础。

Experiment study on root avulsion model of brachial plexus

Objective To introduce a new experimental model for treatment of root avulsion of brachial plexus in rats. Methdos Twenty adult female Wistar rats were divided into two groups. In group one C3 and C4 nerve roots were used to repair C5 and C6. In group two right brachial plexus are thoroughly cut and removed. The electromyologram of two sides' biceps brachii in each group were detected and histological studies (H-E staining) of musculocutaneous nerve were carried out after their sacrifice. Results Three months postoperatively there were a few myelinated fibers to regenating into the repaired roots, and biceps brachii was atropic lightly in the experimental group. And in the denervated rats, nerve fibers degeneration, collapse absorption and myatrophy were apparent. Conclusion The new experimental animal was reasonable to study root avulsion of brachial plexus.