| 应用RNA干扰技术抑制人膀胱癌细胞EGFR基因表达的研究 |
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| 引用本文： | 张朝晖,张志伟,王名伟,黄滔,楚晨龙,赵晨晖,杨安卿,沈周俊,周文龙.应用RNA干扰技术抑制人膀胱癌细胞EGFR基因表达的研究[J].中华肿瘤防治杂志,2011,18(10):755-757,761. |
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| 作者姓名： | 张朝晖 张志伟 王名伟 黄滔 楚晨龙 赵晨晖 杨安卿 沈周俊 周文龙 |
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| 作者单位： | 1. 上海交通大学医学院附属瑞金医院卢湾分院泌尿外科,上海,200020
2. 上海交通大学医学院附属瑞金医院泌尿外科,上海,200025 |
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| 基金项目： | 上海市卫生局青年科研项目(2006Y065) |
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| 摘 要： | 目的:应用RNA干扰(RNAi)技术抑制人膀胱癌细胞(BIU-87)EGFR基因的表达,探索采用该技术干预膀胱癌的可行性.方法:制备3个针对人EGFR基因的shRNA表达质粒,转染BIU-87细胞48 h后,RT-PCR及蛋白质印迹法检测EGFR基因的表达.结果:酶切及测序鉴定证实,3个shRNA表达质粒构建成功,将其...
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| 关 键 词： | 膀胱肿瘤 基因 erbB-1 RNA 小分子干扰 逆转录聚合酶链反应 |
Inhibition of the EGFR gene by RNA interference in the cells of human bladder cancer |
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| ZHANG Zhao-hui,ZHANG Zhi-wei,WANG Ming-wei,HUANG Tao,CHU Chen-long,ZHAO Chen-hui,YANG An-qing,SHEN Zhou-jun,ZHOU Wen-long.Inhibition of the EGFR gene by RNA interference in the cells of human bladder cancer[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(10):755-757,761. |
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| Authors: | ZHANG Zhao-hui ZHANG Zhi-wei WANG Ming-wei HUANG Tao CHU Chen-long ZHAO Chen-hui YANG An-qing SHEN Zhou-jun ZHOU Wen-long |
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| Institution: | ZHANG Zhao-hui1,ZHANG Zhi-wei1,WANG Ming-wei1,HUANG Tao1,CHU Chen-long1,ZHAO Chen-hui1,YANG An-qing1,SHEN Zhou-jun2,ZHOU Wen-long21.Department of Urology,Luwan Branch,Ruijin Hospital,Medical School of Shanghai Jiao Tong University,Shanghai 200020,P.R.China2.Department of Urology,Shanghai 200050,P.R.China |
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| Abstract: | OBJECTIVE: To inhibit the expression of EGFR gene by RNAi in the cells of human bladder cancer(BIU-87),and investigate the feasibility of intervention for bladder cancer.METHODS: Three expression plasmids with shRNAs targeting the EGFR gene of homo sapien were constructed.The recombinant plasmids were transfected into BIU-87 cells.Inhibition of EGFR gene was detected by RT-PCR and Western blot after 48 h.RESULTS:The expression plasmids were constructed successfully.After transfected into BIU-87,EGFR gene wa... |
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