巨噬细胞移动抑制因子在骨肉瘤中的表达及其与预后的关系

目的 探讨巨噬细胞移动抑制因子（MIF）在骨肉瘤中的表达及其与临床病理特征和预后的关系。方法 应用免疫组化法检测61例骨肉瘤组织及15例癌旁组织（距肿瘤组织边缘＞5.0cm）中MIF表达，分析MIF表达与骨肉瘤临床病理特征之间的关系。采用Kaplan-Meier法计算生存情况，Cox多因素回归进行生存分析。结果 骨肉瘤组织中MIF阳性表达率为62.3％，癌旁组织为33.3％，差异有统计学意义（P=0.043）。MIF表达与骨肉瘤患者的性别、年龄、临床分期、组织分型无关，与肺转移相关（P=0.006）。MIF阳性表达组患者的3年生存率为70.3%，显著低于阴性表达组的95.8%（P=0.019）。Cox多因素回归分析显示，Enneking分期是影响患者预后的独立因素，而MIF表达非独立预后因素。结论 MIF表达与骨肉瘤转移密切相关，有助于评价骨肉瘤患者的预后。

Expression of macrophage migration inhibitory factor in osteosarcoma and its correlation with prognosis

Objective To investigate the expression of macrophage migration inhibitory factor（MIF） in osteosarcoma, and explore the relationship between MIF and clinicopathological factors and prognosis. Methods Immunohistochemistry was applied to detect expression of MIF in 61 osteosarcoma tissues and 15 corresponding adjacent tissues（ 〉 5.0cm at the outer edge of the tumor tis- sue）. The relationship between MIF expression and clinicopathological factors of osteosarcoma was evaluated by chi-square test. Sur- vival was calculated by Kaplan-Meier method, and survival analysis was proceeded by Cox regression. Results The positive rate of MIF in osteosarcoma tissues was 62. 3%, and in adjacent tissues was 33.3%. The difference was statistically significant（ P = 0. 043）. The expression of MIF was significantly correlated with lung metastasis （P = 0. 006）, but not related with sex, age, location, clinical stage and pathological type. The three-year survival rate of patients with MIF positive expression was lower than that of those with MIF negative expression（70. 3% vs. 95.8%, P = 0. 019）. Cox proportional hazards regression analysis confirmed that Enneking staging was an independent prognostic facor of osteosarcoma, while MIF was not. Conclusion MIF may play an important role in metastasis in osteosarcoma, so might be employed to evaluate the prognosis of osteosarcoma patients.