1800 MHz长期电磁辐射对NIH/3T3细胞增殖能力的影响

目的探讨长期1800 MHz电磁辐射对NIH/3T3细胞增殖能力的影响。方法采用1800MHz电磁辐射,SAR值2 W/kg,间断暴露方式(5 min on/10 min off),5 h/d。对暴露60 d和138 d的细胞,进行平板克隆和软琼脂克隆实验,并采用ELISA法检测端粒酶的表达。结果平板克隆实验中,60 d暴露组细胞的克隆形成率显著低于假暴露组(P0.05),而138 d暴露组细胞的克隆形成率与假暴露组无明显差异。软琼脂克隆实验中,60 d暴露组和假暴露组细胞均未见克隆形成,而138 d暴露组克隆形成率显著高于假暴露细胞。端粒酶检测结果,60 d暴露组细胞的端粒酶表达量与假暴露组无显著差异,而138 d暴露组细胞的端粒酶表达量显著高于假暴露组(P0.05)。结论 1800 MHz电磁辐射长期暴露至60 d,有损NIH/3T3细胞的增殖能力,而更长期的暴露后(138 d)细胞转化增殖能力增强。

Effects on proliferation ability of NIH/3T3 cells induced by long-term 1800 MHz electromagnetic radiation

Objective To observe the proliferation ability of NIH/3T3 cells after a long-term exposure to 1800 MHz electromagnetic radiation.Methods Cells were exposed to 1800 MHz electromagnetic radiation,at SAR of 2 W/kg,intermittently exposure mode (5 min on/10 min off),5 h/d.Then plate clone forming test and soft agar clone forming test of the exposed and sham exposed cells were cultured respectively after 60-day and 138-day exposure.Telomerase expressions of each group were detected by ELISA.Results In plate clone experiments,plate clone forming ratio was significantly lowcr than that of the sham group (P＜O.05) after 60-day exposure,however,there was no significant difference between the exposed cells and the sham after 138-day exposure.In soft agar clone experiments,soft agar clone forming was no significantly different from sham group after 60-day exposure,however,138-day exposure group formation was significantly higher than that of the sham group (P＜0.05).In telomerase experiments,after 60 days of exposure,the expression of telomerase was no significantly different from sham group,whereas 138-day exposure group was significantly higher than sham group (P ＜ 0.05).Conclusions 1800 MHz electromagnetic radiation could damage the proliferation of NIH/3T3 cells exposed for 60 days,whereas the ability of cell transformation and proliferation increased significantly after more long-term exposure (138 days).