| 海参虫草复合物降血糖及机制研究 |
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| 引用本文： | 柳东,王静凤,薛勇,王玉明,傅佳,薛长湖. 海参虫草复合物降血糖及机制研究[J]. 营养学报, 2011, 33(6) |
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| 作者姓名： | 柳东 王静凤 薛勇 王玉明 傅佳 薛长湖 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,青岛,266003 |
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| 基金项目： | 国家自然基金资助项目(No.30972284); 海洋公益性行业科研专项(No.201105029); 国际科技合作项目(No.2010DFA31330); “泰山学者建设工程专项经费” |
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| 摘 要： | 目的探讨海参虫草复合物(Apostichopus japonicus and Cardyceps millitaris,AC)对糖尿病大鼠的降血糖作用。方法通过一次性腹腔注射链脲佐菌素(streptozocin,STZ)建立糖尿病大鼠模型,将成模大鼠随机分为模型对照组、海参虫草复合物低剂量组、高剂量组。灌胃海参虫草复合物35 d后,分别检测大鼠空腹血糖值和血清胰岛素含量;采用RT-PCR法测定肌肉及脂肪组织中磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)、蛋白激酶(serine-threonine kinases,Akt)和葡萄糖转运蛋白4(glucose transporter 4,Glut4)mRNA的表达,Western blot方法检测肌肉及脂肪中Glut4蛋白的表达。结果海参虫草复合物能显著降低糖尿病大鼠空腹血糖(P<0.01),增加血清胰岛素含量(P<0.01),上调肌肉及脂肪组织中PI3K、Akt和Glut4的mRNA表达(P<0.05或P<0.01),增高脂肪组织中Glut4的蛋白表达水平(P<0.01)。结论海参虫草复合物具有降低血糖的作用,其机制可能与部分上调胰岛素介导的PI3K/Akt/Glut4信号转导通路有关。
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| 关 键 词： | 日本刺参 蛹虫草 磷脂酰肌醇3-激酶 蛋白激酶Akt 葡萄糖转运蛋白4 |
THE HYPOGLYCEMIC EFFECT OF MIXTURE OF APOSTICHOPUS JAPONICUS AND CORDYCEPS MILITARIS AND ITS MECHANISM |
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| LIU Dong , WANG Jing-feng , XUE Yong , WANG Yu-ming , FU Jia , XUE Chang-hu. THE HYPOGLYCEMIC EFFECT OF MIXTURE OF APOSTICHOPUS JAPONICUS AND CORDYCEPS MILITARIS AND ITS MECHANISM[J]. Acta Nutrimenta Sinica, 2011, 33(6) |
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| Authors: | LIU Dong WANG Jing-feng XUE Yong WANG Yu-ming FU Jia XUE Chang-hu |
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| Affiliation: | LIU Dong,WANG Jing-feng,XUE Yong,WANG Yu-ming,FU Jia,XUE Chang-hu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | Objective To study the hypoglycemic activity of the mixture of Apostichopus japonicus and Cordyceps militaris(AC) in diabetic rats.Method Rat diabetic model was induced by a single intraperitoneal injection of streptozotocin.Diabetic rats were randomly divided into diabetic control group,low-dose AC group and high-dose AC group.The diabetic rats were given different dosages of AC daily for 5 w.Each of the following indicators was inspected: the change of the level of fasting blood glucose and serum insulin ... |
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| Keywords: | Apostichopus japonicus Cordyceps militaris phosphatidylinositol 3-kinase serine-threonine kinases |
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