5-羟色胺对人胎盘滋养细胞钙通道的影响

目的探讨5-羟色胺对胎盘滋养细胞钙通道的影响。方法将体外培养的胎盘滋养细胞分成3组,低深度5-羟色胺组(5-TH,1μmol/L)、高浓度5-TH组(10μmol/L)和对照组。采用全细胞膜片钳方法,在胎盘滋养细胞外液中分别加入L-型钙通道阻滞剂、T-型钙通道阻滞剂、钠通道阻滞剂及5-TH,检测其电流值。结果在-60～-50mV电压时,胎盘滋养细胞L-型钙通道开放,-40～-30mV时,其最大峰值电流为(82.31±6.46)皮安(pA)。加入尼莫地平(Nimodpine)可阻断大部分电流。氟桂利嗪(Flunari-zne)和替曲朵辛(TTX)不能抑制该电流成分。加入1μmol/L和10μmol/L5-TH,其峰值电流分别为(104.77±10.84)pA和(117.16±9.67)pA,与对照组比较,差异有统计学意义(P0.05);加入5-TH(1μmol/L)和Nimodpine、5-TH(10μmol/L)和Nimodpine后,峰值电流分别为(85.35±6.54)pA和(89.42±7.62)pA,与对照组比较,差异无统计学意义(P0.05)。结论5-TH可激活胎盘滋养细胞L-型钙通道,促使钙电流值增加。

Influence of 5-HT on calcium channel of human placental trophoblast cell

Objective To investigate the effects of 5-hydroxytryptamine （5-HT） on calcium channel of human placental cytotrophoblast cell（HPCC）. Methods The current characterize of L-type calcium channels and Ca2＋ current of the cultured HPCC were sought by whole cell patch-clamp recordings,The HPCC were divided into 3 groups：control group,lower and higher 5-HT groups （1.0 and 10.0μmol/L）. We add L-type calcium channels antagonnist（10 μmol/L）,T-type calcium channels antagonnist（10 μmol/L）and sodium channels antagonnist（10 μmol/L） in to HPCC before detecting the current. In the same way,We add 5-TH（1 μmol/L） and L-type calcium channels antagonnist （10 μmol/L）,5-TH（10 μmol/L） and L-type calcium channels antagonnist （10 μmol/L） HPCC before detecting the current.Current value was detected.Results The current voltage relate to the current,which reached a maximum at-30～-40mV and was total inactivated at -50～-60mV.This fast inactivating inward current was blocked by Nimodpine.TTX or Flunarizne failed to affect the voltage-dependent inward current. This channel could activated by 5-HT.5-HT（1μmol/L,10μmol/L）could increase the Ca2＋ current and the maximal amplitude reach at （104.77±10.84）pA and （117.16±9.67）pA,which had sigrificant defference compared with control group（P0.05）.The effects of 5-HT increasing the calcium current was significantly blocked by Nimodpine. Conclusion：5-HT may be a L-type calcium channels activating agent which can lead to L-type calcium channels activiting and increasing calcium current.