辅助生殖技术后流产胚胎核型与超声结果分析

目的 探讨辅助生殖技术(ART)治疗后妊娠早期影响胚胎发育的因素.方法 以85例不孕症患者早孕期流产胚胎为研究对象,分析绒毛染色体以及异常核型、不同受孕方式和年龄对超声结果的影响.结果 85例流产胚胎中异常核型占50.6％,全部为染色体数目异常;有胚芽的流产胚胎异常核型率(63.3％)显著高于无胚芽组(33.3％),差异有统计学意义(P=0.006);有胚芽的胚胎由体外受精联合胚胎移植技术(IVF)受精的比例(44.9％)较无胚芽组低(80.6％),差异有统计学意义(P=0.001);流产患者的平均年龄在有胚芽和无胚芽组间分别为(33.4 ± 5.2)岁和(33.0 ± 4.4)岁,但差异无统计学意义(P=0.713).结论 经ART妊娠后胚胎自然流产的异常核型主要是染色体数目异常,出现胚芽的胚胎中染色体异常率较高,IVF受精的胚胎空孕囊的比例较高,年龄对胚胎组织结构的发育无显著影响.

Analysis of karyotype and ultrasound findings in miscarriage embryo following assisted reproductive technology

Objective To analyze the factors affecting embryo development in first trimester following assisted reproductive technolo-gy ( ART) . Methods Spontaneous abortion embryos from 85 conceptions following ART were analyzed by karyotype, ultrasound findings, the mode of conception and age. Results In 85 abortion embryos, abnormal karyotype consisted 50. 6％, all of which were numerical disor-ders;the percentage of abnormal karyotype in embryos with embryonic pole was significantly higher than that without embryonic pole (63. 3％vs 33. 3％, P=0. 006);most embryos with empty sac was fertilized by IVF (80. 6％), which was significantly more than that in embryos with embryonic pole (44. 9％, P=0. 001);between infertile patients with two different embryo morphology, no age effect was found ( P=0. 929). Conclusion Most abnormal karyotype in miscarriage following ART is numerical disorder and contributes more in abortion embryos with embryonic pole. Most embryos with empty sac come from IVF. No age effect is found in different ultrasound findings of abortion embryo.