坐骨神经损伤诱导脊髓IL-1 mRNA的表达及其与星形胶质细胞、NOS阳性细胞的关系

目的 观察坐骨神经损伤不同时间脊髓内ＩＬ－１ ｍＲＮＡ表达及其与星形胶质细胞、ＮＯＳ阳性细胞间的关系。方法 采用坐骨神经切断所致大鼠脊髓前角运动神经元损伤模型,应用ＲＴ－ＰＣＲ和免疫组织化学技术。结果 （１）ＩＬ－１在脊髓内的表达直接与坐骨神经损伤及时程密切相关,损伤３ｄ在受累脊髓节段出现的ＩＬ－１ ｍＲＮＡ的表达,损伤７ｄＩＬ－１表达明显,１４ｄ时表达仍然存在。（２）坐骨神经损伤后,脊髓受累节段

Expression of IL-1 mRNA and its correlation with astrocytes and NOS-positive neurons in rat spinal cord following sciatic nerve injury

OBJECTIVE: To study expression of Interleukin 1 (IL-1) and its correlation with astrocytes and nitric-oxide synthase (NOS)-positive neurons in rat spinal cord following sciatic nerve transection. METHODS: Using RT-PCR and immunohistochemistry methods. RESULTS: The experiments showed that IL-1 expression appeared with a low frequency at 3 days after the axontomy, while it could be markedly detectable on post-lesioned day 7 and 14. In normal controls and the spinal fragments without being involved in the injury, no signal of IL-1 mRNA expression was detected with the same condition. At 7 days after nerve injury, the lesioned side of spinal cord began to show strong glial fibrillary acidic protein (GFAP) immunoreactive astrocytes and induction of NOS in the motoneurons that are normally NOS-negative. Up to 21 days after operation, GFAP staining showed star-shaped astrocytes closely resembling the typical appearance of fibrous astrocytes and GFAP-immunoreactive profiles were often seen surrounding NOS-positive neurons. Analysis of the time course of IL-1 mRNA expression, appearance of NOS-positive neurons and changes of reactive astrocytes suggested a close relationship between them. CONCLUSIONS: IL-1, NO might function as important mediators between injured motoneurons and astrocytes.