三阴性乳腺癌外周血及病灶微环境辅助性和调节性T细胞表达

目的分析三阴性乳腺癌(trip nenegative breast cancer,TNBC)患者外周血及病灶微环境辅助性T细胞和调节性T细胞的表达水平。方法选择2012年1—4月经临床及病理确诊的女性TNBC 34例(TNBC组)、非三阴性乳腺癌(NTNBC)39例(NTNBC组)及同期确诊为乳腺良性疾病女性患者27例(对照组),均检测外周血及病灶微环境辅助性T细胞(CD3+、CD4+、CD8+、CD4+/CD8+)和调节性T细胞(CD4CD25+Treg和CD4+CD25+Foxp3+Treg),并进行比较。结果 TNBC组外周血及病灶微环境CD3+、CD4+水平、CD4+/CD8+比值均明显低于NTNBC及对照组,而CD8+水平则明显高于其他两组(P0.05);TNBC组外周血及病灶微环境CD4CD25+Treg及CD4+CD25+Foxp3+Treg表达水平均明显高于NTNBC组及对照组(P0.05)。结论 TNBC患者存在明确的外周血及病灶微环境调节性T细胞和辅助性T细胞异常表达,其可作为本病免疫学病因诊断及评估疗效的特征性标识,并为今后寻找TNBC免疫治疗途径提供试验数据。

Auxiliary and Regulatory T cells Expression in Peripheral Blood and Lesion Microenvironment of Patients with Triple-negative Breast Cancer

Objective To analyze the levels of the auxiliary and regulatory T cells expression in peripheral blood and lesion microenvironment of patients with triple-negative breast cancer（ TNBC）. Methods The levels of auxiliary T cells（ CD3^＋,CD4^＋,CD8^＋,CD4^＋/CD8^＋） and regulatory T cells（ CD4CD25^＋ Treg and CD4^＋CD25^＋Foxp3^＋Treg） in peripheral blood and lesion microenvironment were detected for 34 TNBC patients（ TNBC group） confirmed by clinical and pathological results,39 non-TNBC patients（ NTNBC group） and 27 patients with benign breast diseases（ control group） from 2012January to April,and the levels in the three groups were also compared. Results In the TNBC group,the levels of CD3^＋,CD4^＋ and CD4^＋/CD8^＋ were significantly lower than those in the NTNBC and control groups,but the CD8＋ level was significantly higher than that in the NTNBC and control groups（ P 〈0. 05）; the levels of CD4CD25＋Treg and CD4＋CD25＋Foxp3＋Treg in the TNBC group were significantly higher than those in the NTNBC and control groups（ P 〈0. 05）. Conclusion The auxiliary and regulatory T cells are abnormally expressed in the peripheral blood and lesion microenvironment in TNBC patients,which is a characteristic parameter of immunology in etiological diagnosis and evaluation effect,wihich may provide test data to find a method of TNBC immunotherapy in the future.