Keratinocytes express functional CARD18, a negative regulator of inflammasome activation,and its altered expression in psoriasis may contribute to disease pathogenesis |
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| Institution: | 1. Department of Dermatology and Allergology, University of Szeged, Korányifasor 6, H-6720 Szeged, Hungary;2. MTA-SZTE Dermatological Research Group, University of Szeged, Korányifasor 6, H-6720 Szeged, Hungary;3. Department of Medical Genetics, University of Szeged, Somogyi u. 4, H-6720 Szeged, Hungary;1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA;2. Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Boston, MA 02115, USA;3. Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA;4. FEI Company, Nanoport Europe, 5651 GG Eindhoven, the Netherlands;5. Institute of Complex Systems, Forschungszentrum Jülich, 52425 Jülich, Germany;6. Physics Department, Heinrich-Heine Universität Düsseldorf, 40225 Düsseldorf, Germany;7. Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA;1. Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea;2. College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Republic of Korea;3. Macrogen Inc., Seoul, 08511, Republic of Korea;1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.;2. Department of Orthodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, China |
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| Abstract: | Caspase recruitment domain family member 18 (CARD18, Iceberg) is known as a negative regulatory molecule that inhibits inflammatory events by terminating inflammasome activation due to a direct interaction with pro-caspase-1.During the investigation of molecular mechanisms in keratinocytes that contribute to the pathogenesis of psoriasis, we found that CARD18 expression differs in healthy and psoriatic skin; moreover, CARD18 demonstrated altered response under inflammatory conditions in healthy and psoriatic skin. In healthy skin, low basal CARD18 expression was detected, which showed significant elevation in response to inflammatory stimuli (lymphokine treatment or mechanical injury). In contrast, higher basal expression was observed in psoriatic non-involved skin, but no further induction could be detected.We demonstrated that keratinocytes express CARD18 both at mRNA and protein levels and the expression increased in parallel with differentiation. The investigation of cellular inflammatory processes revealed that psoriasis-associated danger signals triggered the expression of inflammasome components (AIM2, Caspase-1) and CARD18 as well as IL-1β production of keratinocytes. Furthermore, gene-specific silencing of CARD18 in cells treated with cytosolic DNA (poly(dA:dT)) resulted in increased IL-1β secretion, suggesting a negative regulatory role for CARD18 in keratinocyte inflammatory signaling.The differential regulation of CARD18 in healthy and psoriatic uninvolved epidermis may contribute to the susceptibility of psoriasis. Furthermore, our in vitro results indicate that CARD18 may contribute to the fine tuning of keratinocyte innate immune processes. |
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| Keywords: | Psoriasis Inflammation Caspase-1 AIM2 IL-1β CARD18 |
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