Expression of CXCL4 and aquaporin 3 and 10 mRNAs in patients with otitis media with effusion |
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| Affiliation: | 1. Department of Otorhinolaryngology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China;2. Department of Pediatrics, School of Medicine, Kyung Hee University, Seoul, Republic of Korea;3. Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Republic of Korea;4. Department of Otorhinolaryngology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea;1. Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, Sweden;2. Division of Oncology, Department of Clinical Sciences, Lund University, Lund, Sweden;3. Division of Neuroradiology, Department of Clinical Sciences, Lund University, Lund, Sweden;1. Wonkwang University Hospital, Iksan, South Korea;2. Cardiovascular Research Foundation, New York, New York;3. Helios Amper-Klinikum, Dachau, Germany;4. Columbia University Medical Center, New York, New York;5. Wellmont CVA Heart Institute, Kingsport, Tennessee;6. Sanger Heart & Vascular Institute/Carolinas HealthCare System, Charlotte, North Carolina;7. The Ohio State University Wexner Medical Center, Columbus, Ohio;8. Reid Heart Center, FirstHealth of the Carolinas, Pinehurst, North Carolina;9. Shaare Zedek Medical Center, Jerusalem, Israel;10. LeBauer Cardiovascular Research Foundation/Cone Health, Greensboro, North Carolina;2. Division of Radiology, Ribeirao Preto Medical School, University of Sao Paulo, São Paulo, Brazil;3. Case Western Reserve University, Cleveland, OH;4. Cleveland, OH;1. Division of Urology, Kobe University Graduate School of Medicine, Kobe, Japan;2. Department of Urology, Faculty of Medicine, Assiut University, Assiut, Egypt |
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| Abstract: | PurposeBacterial infections in children with underdeveloped Eustachian tubes are a major cause of otitis media with effusion (OEM), and persistent effusion in the middle ear in these patients is a major cause of surgical intervention. CXCL4 is associated with bacterial infection, and aquaporins 3 and 10 are associated with water metabolism. This study assessed the expression of mRNAs encoding CXCL-4 and aquaporins 3 and 10 in the effusion of pediatric OME patients, and the association of this expression with clinical manifestations.MethodsLevels of CXCL4 and aquaporin 3 and 10 mRNA were assayed by real-time RT-PCR in the middle ear effusion of 38 pediatric patients with OME requiring ventilation tube insertion. The relationships of these mRNA levels with the presence of bacteria; concomitant diseases such as allergic rhinitis, sinusitis, and adenoid disease; recurrence of OME; and number of ventilation tube insertions were evaluated.ResultsCXCL4 and aquaporin 3 and 10 mRNAs were expressed in middle ear effusion of all OME patients. CXCL-4 mRNA levels were significantly lower when bacteria were present and in patients with concomitant diseases (p < 0.05 each). Levels of all three mRNAs were unrelated to OME recurrence or number of ventilation tube insertions (p > 0.05 each). The levels of CXCL4 and aquaporin 10 mRNAs were significantly correlated (p < 0.05).ConclusionExpression of CXCL4 and aquaporin 3 and 10 mRNAs in middle ear effusion is associated with the pathophysiology of OME. CXCL4 mRNA levels are significantly lower in patients with than without concomitant diseases or bacterial infections. |
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| Keywords: | Otitis media with effusion (OME) CXCL4 and 7 Aquaporin 3 and 10 |
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