Dual oxidases participate in the regulation of intestinal microbiotic homeostasis in the kuruma shrimp Marsupenaeus japonicus |
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| Institution: | 1. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia;2. Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt;3. Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt;4. Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt;5. Arid Lands Agricultural Research Institute, Ain Shams University, Cairo, Egypt;6. School of Agriculture, Purdue University, West Lafayette, Indiana, USA;7. Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3. Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;1. College of Fisheries, Guangdong Ocean University, Zhanjiang, PR China;2. MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;3. Institute of Aquatic Economic Animals, Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China;4. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China;5. School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China;6. South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China |
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| Abstract: | The metazoan gut lumen harbors numerous microbial communities. Tolerance for high bacterial counts and maintenance of microbiota homeostasis remain insufficiently studied. In this study, we identified a novel dual oxidase (MjDUOX2) involved in reactive oxygen species (ROS) production in the kuruma shrimp Marsupenaeus japonicus. MjDUOX2 is a transmembrane protein with an N-signal peptide region (19 aa) and a peroxidase homology domain (PHD, 554 aa) in the extracellular region; seven transmembrane regions; and three EF (calcium-binding region) domains (110 aa), a FAD-binding domain (104 aa), and a NAD-binding domain (156 aa) in the intracellular region. The novel MjDUOX2 exhibits a relatively low similarity (26.84% identity) to a previously reported DUOX in the shrimp (designated as MjDUOX1). The mRNA of MjDUOXs was widely distributed in the hemocytes, heart, hepatopancreas, gills, stomach, and intestine. Oral infection of the shrimp with pathogenic bacteria upregulated the mRNA expression of MjDUOXs and increased the ROS level in the intestine. However, High ROS level could inhibit the expression of MjDUOXs in shrimp after Vibrio anguillarum infection. Knockdown of MjDUOXs by RNA interference (RNAi) decreased the ROS level, increased the bacterial count in the intestine, and decreased the survival rate of the MjDUOX-RNAi shrimp infected with V. anguillarum. These results suggest that MjDUOXs play an important role for microbiota homeostasis in intestine of shrimp. |
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| Keywords: | Intestinal innate immune Reactive oxygen species Dual oxidase Bacteria population |
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