Abnormal labyrinthine zone in the Hectd1-null placenta |
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| Affiliation: | 1. Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Anhui Medical University, Hefei, China;2. Department of Histology and Embryology, Anhui Medical University, Hefei, China;3. Second Affiliated Hospital, Anhui Medical University, Hefei, China;1. Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai, China;2. CITIC Medical & Health Group, Beijing, China;3. Department of Health Management, Jiangxi University of Traditional Chinese Medicine, China;4. Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China;5. State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, China;1. Faculty of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia;2. School of Pharmacy, The University of Jordan, Amman 11942, Jordan;3. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia;4. School of Medical Sciences, Faculty of Medicine, The University of New South Wales, Sydney, NSW 2052, Australia;5. Anzac Research Institute, The University of Sydney, Concord, NSW 2139, Australia |
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| Abstract: | IntroductionThe labyrinthine zone of the placenta is where exchange of nutrients and waste occurs between maternal and fetal circulations. Proper development of the placental labyrinth is essential for successful growth of the developing fetus and abnormalities in placental development are associated with intrauterine growth restriction (IUGR), preeclampsia and fetal demise. Our previous studies demonstrate that Hectd1 is essential for development of the junctional and labyrinthine zones of the placenta. Here we further characterize labyrinthine zone defects in the Hectd1 mutant placenta.MethodsThe structure of the mutant placenta was compared to wildtype littermates using histological methods. The expression of cell type specific markers was examined by immunohistochemistry and in situ hybridization.ResultsHectd1 is expressed in the labyrinthine zone throughout development and the protein is enriched in syncytiotrophoblast layer type I cells (SynT-I) and Sinusoidal Trophoblast Giant cells (S-TGCs) in the mature placenta. Mutation of Hectd1 results in pale placentas with frequent hemorrhages along with gross abnormalities in the structure of the labyrinthine zone including a smaller overall volume and a poorly elaborated fetal vasculature that contain fewer fetal blood cells. Examination of molecular markers of labyrinthine trophoblast cell types reveals increased Dlx3 positive cells and Syna positive SynT-I cells, along with decreased Hand1 and Ctsq positive sinusoidal trophoblast giant cells (S-TGCs).DiscussionTogether these defects indicate that Hectd1 is required for development of the labyrinthine zonethe mouse placenta. |
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| Keywords: | HECT E3 ligase Placenta Labyrinthine layer IUGR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" Intrauterine growth restriction TGC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" trophoblast giant cell H&E" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" Hematoxylin and eosin |
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