抗眼镜王蛇毒卵黄抗体的免疫活性及其对眼镜王蛇毒的中和作用

目的：探讨抗眼镜王蛇毒卵黄抗体的免疫活性及其在体内外对眼镜王蛇毒的中和作用。方法：采用嗜硫色谱法一步分离纯化抗眼镜王蛇毒卵黄抗体,通过间接ELISA法对制备的抗眼镜王蛇毒卵黄抗体进行免疫活性检测,采用小鼠体内外保护实验评估抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒的中和作用。结果：嗜硫色谱法制备的卵黄抗体具有良好的免疫活性,并且对眼镜王蛇毒素显示较好的中和效应,在体外6mg/kg抗眼镜王蛇毒卵黄抗体可有效地中和约4LD50（约1.6mg/kg）的眼镜王蛇毒素,而在体内可有效地中和约3LD50（约1.2mg/kg）的眼镜王蛇毒素。结论：抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒具有良好的中和作用,本项目为抗眼镜王蛇毒卵黄抗体的进一步研究开发提供实验依据。

Immunoreactivity and neutralization against king cobra venom of anti-king cobra venom IgY

AIM: To explore the immunoreactivity and neutralization against king cobra venom of anti-king cobra venom IgY. METHODS: The anti-king cobra venom IgY was developed by thiophilic adsorption chromatography .The biological activity of anti-king cobra venom IgY was detected by indirect ELISA. In vivo and in vitro protective experiments in mice were used for evaluating the neutralization against king cobra venom of anti-king cobra venom IgY. RESULTS: The anti-king cobra venom IgY developed by thiophilic adsorption chromatography showed good biological activity and immunoreactivity,and it also neutralized king cobra venom well. A dose of 6 mg/kg anti-king cobra venom IgY could neutralize 4 LD50(about 1.6 mg/kg)of king cobra venom in vitro, and neutralize 3 LD50(about 1.2 mg/kg)of king cobra venom in vivo. CONCLUSION: The anti-king cobra venom IgY can neutralize king cobra venom,and the present study provides experiment data for further study of anti-king cobra venom IgY.