前列腺组织中EGF、bFGF的表达

目的：研究EGF、bFGF在前列腺组织中的表达。方法：应用mRNA斑点杂交、原位杂交、免疫组织化学及原位杂交与免疫组织化学双染法检测6例正常前列腺（NP）、27例良性前列腺增生症（BPH）前列腺组织中EGF及bFGF的表达。结果：BPH前列腺组织和NP组织中无均无EGF mRNA表达，EGF蛋白表达呈弱阳性，两组间差异无显著性意义（P＞0.05）；NP组织上皮细胞有较多bFGF mRNA表达，但无bFGF翻译，基底基质细胞有少量mRNA及蛋白表达,二者表达水平基本一致；BPH前列腺组织上皮细胞无bFGF mRNA表达，但局灶性增殖上皮细胞细胞膜上有bFGF，基底基质细胞有大量bFGF mRNA转录及蛋白质翻译，以局灶性增殖区最为明显。结论：NP及BPH的前列腺组织中无EGF分泌细胞；bFGF在前列腺基底基质细胞过度表达，以自分泌、旁分泌方式促进了基质和上皮的非均一性增殖。

Expressions of EGF and bFGF in human prostate

Purpose:To study the expression and functional way of EGF and bFGF in human prostatic tissue.Method:To detect the expression of EGF and bFGF in 6 cases of human normal prostate (NP) and 27 cases of benign prostatic hyperplasia (BPH) specimens using mRNA dot blot, in situ hybridization and immunohistochemistry.Results:There was no expression of EGF mRNA in any of the prostate exmined,weakly immunohistochemical staining of EGF was observed in 2 cases of 6 NP and 5 of 27 BPH sepcimens,there was no statistic difference between NP and BPH (P＞0.05).The total amount of bFGF mRNA in BPH tissue is much than that in NP tissue,aboundant bFGF mRNA was revealed in epithelial cells of 6 cases of NP tissues,but no bFGF;low amount of bFGF was expressed,according with the level of its mRNA both in basal and stromal cells of NP tissues;No signal for bFGF mRNA was found in epithelial cells of BPH tissues but bFGF protein was found on surface of local proliferating epithelial cells.Markedly increased expression of bFGF mRNA and its protein were present in basal and stromal cells of BPH tissues,especially in the region of local proliferating stromal cells.Conclusion:There are no EGF secretory cells in human normal or hyperplastic prostate; overexpression of bFGF in basal and stromal cells of human prostate caused irregular hyperplasia both of stromal elements and glandular epithelium via autocrine and paracrine pathways.