电离辐射对小鼠脾淋巴细胞IL-10表达的影响

目的 观察75mGy、2GyX射线全身照射对小鼠脾淋巴细胞中IL－10的影响。方法 采用Northern blot检测IL－10转录水平的变化，并同时通过流式细胞术检测其蛋白合成的变化。结果 ①蛋白合成结果表明，75mGy X射线全身照射后脾淋巴细胞IL－10合成在照后2h开始即呈时间依赖性降低，至48h仍无恢复迹象（P＜0．01），而2Gy照射后则出现IL－10合成升高，于照射后8h达到峰值（P＜0．05）。②Northern blot结果表明，75mGy照射后脾细胞IL－10 mRNA转录水平从照后1h即降低，并维持较低水平一直到48h开始恢复，达到假照射组的88．35％；2GyX射线全身照射后早期mRNA水平即有所升高，2h达到假照组的134．06％，4h略有下降，随后逐渐恢复至假照射组水平。结论 不同剂量X射线全身照射可引起不同的辐射效应，IL－10在其中起着重要的作用。

Changes of IL-10 expression in splenocytes after X-irradiation of different doses

Objective To observe the time course changes in the expression of IL-10 in splenocytes after whole body irradiation (WBI) with different doses of X-rays. Methods Northern blot and flow cytometry(FCM) were adopted to detect the changes of IL-10 at mRNA and protein levels respectively. Results FCM data indicated IL-10 protein synthesis decreased beginning from 2 h after exposure and remained at low level up to 48 h after 75 mGy irradiation.The increase of IL-10 synthesis was detectable after 2 Gy irradiation.By northern blot we found IL-10 mRNA decreased significantly after WBI with 75 mGy X-rays(low dose radiation,LDR),while it increased at 2 h after WBI with 2 Gy X-rays (high dose radiation,HDR) . Conclusion The results suggest LDR and HDR have different effects on IL-10.;