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The effects of thiosemicarbazide on development in the wood frog, Rana sylvatica. II. Critical exposure length and age sensitivity
Authors:E E Riley  M R Weil
Affiliation:1. UK Centre for Ecology & Hydrology, Bush Estate, Penicuik EH26 0QB, UK;2. Institute of Life and Earth Sciences, School of Energy, Geoscience, Infrastructure and Society, Heriot-Watt University, Edinburgh EH14 4AS UK;3. School of Health and Life Sciences, University of the West of Scotland, Paisley PA1 2BE, UK;4. Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK;1. Department of Zoology, Institute of Biological Sciences, University of Zielona Góra, Profesora Zygmunta Szafrana 1, 65-516 Zielona Góra, Poland;2. Doctoral School of Exact and Technical Sciences, University of Zielona Góra, al. Wojska Polskiego 69, 65-762 Zielona Góra, Poland;3. Department of Ecology and Anthropology, Institute of Biology, University of Szczecin, Wąska 13, 71-412 Szczecin, Poland
Abstract:Wood frog (Rana sylvatica) tadpoles were exposed to 50 mg thiosemicarbazide (TSC)/liter water for varying lengths of time and at different developmental stages. Short exposure periods (3 and 6 hr) resulted in no visible deformities. Exposure periods of 12 or more hr caused slight to severe abnormalities, with a direct relationship between length of exposure and degree of deformity. Tadpoles exposed to TSC from posthatching Days 24-30 were more seriously affected than were tadpoles exposed at an older age.
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