腺病毒介导的FasL基因转移及对胃癌细胞生长和凋亡的影响

目的：探讨FasL基因对人胃癌的作用以及FasL基因治疗胃癌的可能性。方法：通过腺病毒载体介导FasL基因转移到低水平表达FasL的人胃癌细胞系SGC－7901，对FasL基因的表达，细胞生长的抑制与机制和对肿瘤生长模型的治疗效果进行分析。结果：外源性FasL基因在靶细胞高水平的表达，显著抑制了SGC－7901的生长和集落形成，流式细胞仪检测显示细胞G1期阻滞并发生了凋亡。瘤内注射Ad-FasL对裸鼠体内移植瘤有一定的抑瘤作用。结论：FasL基因参与了肿瘤细胞凋亡的诱导，能抑制胃癌细胞的生长，因此该基因在肿瘤的基因治疗上有着广泛的应用前景。

Effects on Human Gastric Carcinoma Cell Line SGC-7901 by FasL gene Transduction

Objective:To study the effect of FasL gene on human adenocarcinoma cell line and possibility of exogenous FasL gene for gene therapy of gastric cancer.Methods:An aden-oviral expression vector with full length cDNA of FasL gene was constructed(Ad-FasL)and trans-fected into SGC-7901cells.The effect of exogenous FasL gene on the growth of SGC-7901was examined in vitro and vivo.Results:Expression of FasL gene in SGC-7901cells was con-firmed by FCM and RT-PCR.That in vitro growth of the Ad-FasL transfected SGC-7901cells was completely inhibited(inhibation rate84%)as compared to mock(Ad-LacZ)transfected SGC-7901cells.Colony-forming activity in vitro of the Ad-FasL transfected SGC-7901was complete-ly inhibited.The Ad-FasL transfected cells appeared apoptotic which was confirmed by the ap-pearance of pre-G1on flow cytometry(FCM).The growth of SGC-7901xenografts in nude mice was retarded by intra-tumoral injection of Ad-FasL.Conclusion:FasL gene participates in the induction of cell apoptosis.It is promising to use it for gene therapy of cancer.