Isolation of endothelial cells from human first trimester decidua using immunomagnetic beads. |
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Authors: | B L Drake Y W Loke |
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Affiliation: | Department of Cellular and Genetic Pathology, University of Cambridge, UK. |
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Abstract: | A technique was developed for the isolation of purified endothelial cells from first trimester decidual tissue, using QB End/40-coated magnetic polystyrene beads (Dynabeads). QB End/40 is an endothelial-specific monoclonal antibody which reacts with the coagulation cascade co-factor, thrombomodulin. Small endothelial cell aggregates were released from first trimester decidua by the sequential use of collagenase and trypsin. This dissociation method yielded 15-20% endothelial cells which were further purified to greater than 90% homogeneity by rosetting with QB End/40-Dynabeads. Cultures of purified decidual endothelial cells provide a useful tool for investigating cell-cell interaction in the first trimester placental bed. |
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Keywords: | endothelium/decidua/Dynabeads |
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