Comparative Evaluation of PCR and Imprint Smear Microscopy Analyses of Skin Biopsy Specimens in Diagnosis of Macular,Papular, and Mixed Papulo-Nodular Lesions of Post-Kala-Azar Dermal Leishmaniasis |
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| Authors: | Neena Verma Dharmendra Singh Krishna Pandey Vidya Nand Rabi Das Chandra Shekhar Lal Rakesh Bihari Verma Prabhat Kumar Sinha Pradeep Das |
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| Affiliation: | Department of Pathologya;Department of Molecular Biologyb;Department of Clinical Medicinec;Department of Clinical Biochemistryd;Department of Biostatistics and Epidemiology,e Rajendra Memorial Research Institute of Medical Sciences, Indian Council of Medical Research, Agamkuan, Patna, Bihar, India |
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| Abstract: | Diagnosis of post-kala-azar dermal leishmaniasis (PKDL), particularly the macular form, is difficult when based on microscopy. This study compared the results of nested PCR (91.9% positive samples) with imprint smear microscopy (70.9% positive samples) for 62 PKDL samples. We found that nested PCR, which indicated 87.5% positivity for the macular lesions, compared to 41.6% positivity by imprint smear microscopy, is an efficient method for early diagnosis of PKDL. |
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