Evaluation of Pneumococcal Serotyping by Multiplex PCR and Quellung Reactions |
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| Authors: | Sandra S. Richter Kristopher P. Heilmann Cassie L. Dohrn Fathollah Riahi Daniel J. Diekema Gary V. Doern |
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| Affiliation: | Cleveland Clinic, Cleveland, Ohio, USAa;University of Iowa Carver College of Medicine, Iowa City, Iowa, USAb |
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| Abstract: | Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach. |
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