三七药材的指纹图谱

目的建立三七药材的高效液相指纹图谱分析方法。方法采用Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈水梯度洗脱(体积比为2∶8~8∶2),流速为1.0 mL.min-1,柱温40℃,检测波长203 nm;以人参皂苷Rg1为参照物。结果通过对11批云南省三个地区不同规格的三七样品的测定,标定了15个共有峰,并通过“中药指纹图谱计算机辅助相似度软件”,计算出其相似度均在0.99以上。结论该方法可为更好地控制三七药材的内在质量提供科学依据。

Fingerprint of Radix notoginseng

Objective To establish the method of fingerprint analysis of Radix notoginseng by HPLC.Methods Kromasil C_(18)(4.6 mm×250 mm,5 μm)column was used.The mobile phase was composed of acetonitrile and water with a linear gradient elution.The flow rate was 1.0 mL·min~(-1) with DAD detected at 203 nm.The column temperature was maintained at 40 ℃.Results By the determination of the 11 notoginseng of different specifications gathered from different places,we have pinpointed 15 common peaks.The similarities were calculated and proved all above 0.99 with the help of the similarity calculation soft of fingerprint chromatography of traditional Chinese medicine.Conclusions This method is accurate and reliable.It provides a scientific basis for controlling the quality of Radix notoginseng.