| 肾母细胞瘤RASSF1A基因启动子区甲基化研究 |
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| 引用本文： | 张宝欣,王常林,高红,蔡炜嵩.肾母细胞瘤RASSF1A基因启动子区甲基化研究[J].中国小儿急救医学,2006,13(3):250-252. |
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| 作者姓名： | 张宝欣 王常林 高红 蔡炜嵩 |
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| 作者单位： | 1. 中国医科大学附属第二医院儿外科,辽宁,沈阳,110004
2. 中国医科大学附属第二医院卫生部先天畸形实验室,辽宁,沈阳,110004 |
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| 基金项目： | 辽宁省科学技术基金资助项目（99225015） |
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| 摘 要： | 目的了解肾母细胞瘤RASSF1A基因启动子区甲基化情况。方法手术获取9例肾母细胞瘤及瘤旁残留肾组织,提取DNA后用亚硫酸氢钠处理,巢式PCR扩增RASSF1A基因启动子区DNA片段,测序,检测启动子区甲基化情况。结果发现1例16个CpG岛位点中有10个存在甲基化(10/16),相应瘤旁残留肾组织无甲基化(0/16);在另1例瘤组织中检测到12个CpG岛位点存在甲基化(12/16),但仅有8个CpG岛位点与上例相同,另外4个位点不同。结论肾母细胞瘤中RASSF1A基因启动子区存在甲基化,与瘤旁残留肾组织甲基化情况有差别。
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| 关 键 词： | 肾母细胞瘤 RASSF1A基因 甲基化 |
| 文章编号： | 1673-4912(2006)03-0250-03 |
| 收稿时间： | 2006-04-17 |
| 修稿时间： | 2006-05-08 |
RASSF1A promoter region CpG methylation in Nephroblastoma |
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| ZHANG Bao-xin, WANG Chang-lin, GAO Hong, et al.RASSF1A promoter region CpG methylation in Nephroblastoma[J].Chinese Pediatric Emergency Medicine,2006,13(3):250-252. |
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| Authors: | ZHANG Bao-xin WANG Chang-lin GAO Hong |
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| Institution: | Sheng, jing Hospital affiliated to China Medical University, Shenyang 110004, China |
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| Abstract: | Objective To comprehend the methylated condition of the RASSF1A promoter region in nephroblastoma.Methods Nine cases with nephroblastoma and adjacent tumor kidney tissue DNA were treated by bisulfite,the bisulfited-DNA were amplified for RASSF1A gene fragment with nested polymerase chain reaction,then to sequence the products.Results Finded one case,10 sites had been methylated in 16 sites(10/16),but the correspondent adjacent kidney tumor tissue had no methylation(0/16).Twelve sites had methylation in another case too(12/16),but only 8 sites were the same as the case above,the left 4 sites were different.Conclusion RASSF1A promoter region existed methylation in nephroblastoma and it had differnce between nephroblastoma and adjacent tumor tissue. |
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| Keywords: | Nephroblastoma RASSF1A gene Methylation |
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