| Effect of Cytokines Secreted by Human Adipose Stromal Cells on Endothelial Cells |
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| 引用本文： | 李宾公,曾秋棠,王红祥,毛晓波. Effect of Cytokines Secreted by Human Adipose Stromal Cells on Endothelial Cells[J]. 华中科技大学学报(医学英德文版), 2006, 26(4): 396-398. DOI: 10.1007/s11596-006-0403-0 |
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| 作者姓名： | 李宾公 曾秋棠 王红祥 毛晓波 |
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| 作者单位： | Department of Cardiology Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Hematology，Union Hospital of Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China |
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| 摘 要： | Recently, several observations have pointed to the presence of a population named adipose-derived adult stem cells (ADAS) or adipose stromal cells (ASCs) in human adipose tissue. Subsequent studies revealed that ASCs were multipotent, differentiating along the cardiac myocyte, endothelial, neuronal, and other cells lineages[1, 2], and could secrete cytokines such as HGF and VEGF[3]. Since adipose tissue is an abundant, accessible, and re- plenishable source of adult stem cells that can b…
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| 关 键 词： | 细胞浆移动 脂肪基质细胞 内皮细胞 细胞研究 |
| 收稿时间： | 2006-01-02 |
Effect of cytokines secreted by human adipose stromal cells on endothelial cells |
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| Bingong Li,Qiutang Zeng,Hongxiang Wang,Xiaobo Mao. Effect of cytokines secreted by human adipose stromal cells on endothelial cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2006, 26(4): 396-398. DOI: 10.1007/s11596-006-0403-0 |
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| Authors: | Bingong Li Qiutang Zeng Hongxiang Wang Xiaobo Mao |
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| Affiliation: | 1. Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Hematology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary To isolate and culture adipose stromal cells (ASCs), and study the effect of cytokines secreted by ASCs on endothelial cells, human adipose tissue was digested with collagenase type I solution and ASCs were derived by culture. The cells surface phenotype was examined by flow cytometry. ELISA was used to detect the secretion of VEGF, HGF, SDF-1α and RT-PCR was employed to detect the expression of their mRNA. Then the ASC medium was utilized to culture human umbilical vein endothelial cells ECV304. Cells were counted by hemacytometer to determine the proliferation and Annexin V / PI was employed for the examination of the apoptosis rate of ECV304. ASCs were derived by culture and expressed CD34, CD105 while they did not express CD31 or CD45. ASCs secreted cytokines such as VEGF, HGF and SDF-1α so the ASC medium could stimulate proliferation and counteract apoptosis of endothelial cells (P<0.05). Bcl-2 mRNA was also found to be up-regulated in the endothelial cells. It is concluded that ASCs can secrete cytokines and has significant effect on the proliferation of endothelial cells and apoptosis. LI Bingong, male, born in 1972, Doctor in Charge |
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| Keywords: | adipose stromal cells cytokines endothelial cells |
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