食管鳞癌G3BP和骨桥蛋白的表达及其对患者预后的影响

目的研究食管鳞癌组织中GTP酶激活蛋白SH3功能区结合蛋白(G3BP)和骨桥(OPN)蛋白的表达及其与食管鳞癌生物学行为之间的关系。方法采用免疫组织化学方法检测80例食管鳞癌组织中G3BP和OPN蛋白的表达情况,探讨它们与食管鳞癌的肿瘤大小、组织分化程度、TNM分期、淋巴结转移和预后的关系。结果(1)G3BP蛋白在食管鳞癌组织中的阳性表达率为71.3%,它在淋巴结转移组的阳性表达率高于无淋巴结转移组(Z=-2.283,P=0.022);而与肿瘤大小、组织分化程度、TNM分期无关(P>0.05);G3BP蛋白阳性表达组患者的术后生存时间较阴性表达组短,差异有统计学意义(P=0.000)。(2)OPN蛋白在食管鳞癌组织中的阳性表达率为100%,OPN蛋白的表达水平与组织分化程度(X~2=10.766,P=0.005)及淋巴结转移(Z=-2.289,P=0.022)有关,而与肿瘤大小和TNM分期无关(P>0.05);OPN蛋白的表达水平越高,患者术后生存时间越短(P=0.000)。(3)G3BP蛋白和OPN蛋白在食管鳞癌组织中的表达之间存在正相关性(r(?)=0.376,P=0.001)。结论食管鳞癌组织G3BP和OPN蛋白的表达与淋巴结转移情况及患者的预后有密切关系。

Expression of Ras-GTPase-activiting protein SH 3 domain binding protein and osteopontin in esophageal squamous carcinoma and their effects on prognosis

Objective To investigate the expressions and significances of Ras-GTPase-activiting protein SH 3 domain binding protein(G3BP) and osteopontin (OPN) proteins in esophageal squamous carcinoma (ESC).Methods The expressions of G3BP and OPN proteins in 80 cases of ESC were detected by immunohistochemistry.The relationships between the 2 protein expression and tumor size, differentiation degree,TNM stage,lymph node metastasis and prognosis of ESC were also explored. Results (1)The positive expression rate of G3BP in ESC was 71.3%,and the rate in lymphoid metastatic group was significantly higher than that in non lymphoid metastatic group (Z=-2.283, P=0.022),but no relations were found between G3BP expression and diameter of tumor,differentiation and TNM grade (P>0.05).The G3BP positive expression group had shorter survival time than G3BP negative expression group (P=-0.000).(2)The positive expression rate of OPN in ESC was 100%,and the degree of OPN expression was correlated with the differentiation (X~2=10.766,P=0.005) and lymphoid metastasis (Z=-2.289,P=-0.022),but no relationship was found between the diameter of tumor and TNM grade (P>0.05).The expression of OPN were significantly related to survivals in a negative time-dependent manner in ESC patients (P=0.000).(3)The expression of G3BP protein correlated positively with the degree of OPN expression in ESC tissue (r,=0.376,P=0.001).Conclusions The expressions of G3BP and OPN proteins have a close relationship with lymphoid metastasis and survival in ESC patients.G3BP and OPN proteins can be considered as predictors of prognosis in ESC patients.