评价两种基质联合检测抗核抗体的临床意义

目的 :探讨猴肝组织和人类上皮细胞 (Hep - 2细胞 )检测血清抗核抗体 (ANA)的临床意义。方法 :以猴肝组织和Hep - 2细胞为基质 ,用间接免疫荧光 (IFA)法测 118例结缔组织病患者 ,80例非结缔组织病患者和5 0例健康人血清ANA。结果 :Hep - 2细胞检测ANA阳性率高于猴肝组织检测ANA阳性率 ,其阳性率比较差异有显著性意义 (P <0 .0 5 ) ,两种基质联合检测结缔组织病患者和非结缔组织病患者及健康人组血清ANA阳性率差异有显著性意义 (P <0 .0 1) ,非结缔组织病患者及健康人组血清ANA阳性率差异无显著性意义 (P >0 .0 5 )。结论 :对每一份血清进行两种基质联合检测ANA及ANA滴度的观察对系统性红斑狼疮(SLE)的诊断及疗效的判断有一定的临床意义。

CLINICAL SIGNIFICANT BY TWO SUBSTRATE FOR DETECTION OF ANTINUCLEAR ANTIBODIES IN SERUM

Objective:To study monkey liver tissue and human epithelial cells (Hep-2) as substrate for detection of antinuclear antibodies(ANA) in clinical significance. Methods:Monkey liver tissue and Hep-2 cells were compared as substrates for detection of ANA in the serum of 118 patients with different connective tissue disease(TCD),80 patients with non-CTD and 50 normal subjects ,using indirect immunofluorescence assay. Results:ANA on Hep-2 cells produced more positive results than ANA on monkey liver tissue ,and the difference between them was statistically significant (P<0.05). Compared with the groups of non-TCD and normal,the positive rate of ANA on monkey liver tissue and Hep-2 cells in the groups of TCD was significant different(P<0.01), and there was no significant difference between the groups of non-TCD and normal (P>0. 05).Conclusion:ANA should be detected by monkey liver tissue and Hep-2 cells. Observation titre of ANA have clinical significant in patients with SLE.