Tricyclic antidepressant radioreceptor assay |
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| Authors: | R B Innis L Tune R Rock R Depaulo D C U'Prichard S H Snyder |
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| Affiliation: | 1. Departments of Pharmacology and Experimental Thereapeutics and Psychiatry and Behavioral Science, Baltimore, Maryland 21205, U.S.A.;2. Department of Laboratory Medicine, Hohns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A. |
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| Abstract: | A radioreceptor assay for tricyclic antidepressants described here is based on the ability of these drugs to compete with [3H]-3-quinuclidinyl benzilate (3H-QNB) for binding to muscarinic cholinergic receptors in rat brain membranes. The assay is sensitive, in that in can detect, for example, 2 ng/ml nortriptyline in plasma. Seven plasma samples from depressed patients treated with nortriptyline were assayed with the radioreceptor and gas liquid chromatographic methods, and the results from these two methods were almost identical. This assay should be used cautiously, if at all, in patients treated with other drugs that have potent anticholinergic effects. |
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| Keywords: | Tricyclic antidepressant Radioreceptor assay Anticholinergic QNB displacement |
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