B细胞单克隆形成与幽门螺杆菌感染

为探讨淋巴细胞性胃炎是否有 B细胞单克隆形成 ,以及该克隆形成的意义及其与幽门螺杆菌(HP)感染的关系。采用 Giem sa染色 ,免疫组化染色以及半巢式 PCR技术对 2 6例淋巴细胞性胃炎及 11例对照标本进行 HP检查和 B细胞单克隆性检测。结果显示 :在 2 6例淋巴细胞性胃炎中有 l4例查见 HP,其检出率为5 3.8% ,但与对照组比较无差异 (P>0 .0 5 )。免疫组化发现 2 6例淋巴细胞性胃炎均为多克隆性 ,但 PCR技术却发现有 7例存在 B细胞单克隆性重排 (2 6 .9% ) ,且 HP阳性组其单克隆性重排检出率明显高于 HP阴性组 (P <0 .0 5 )。结果提示 ,组织学及免疫组化显示良性的淋巴细胞性胃炎中确有 B细胞单克隆形成 ,该单克隆的形成可能是肿瘤早期病变的潜在标志 ,且与 HP感染有关 ,但并非仅与 HP感染有关。

B-cell Monoclonality and Infection with Helicobacter plyori

The objectives of this study were to determine whether B cell monoclonal populations may be detected in lymphocytic gastritis, to address the significance of the monoclonality, and to investigate the relation between B cell monoclonality and H.pylori infection. Giemsa staining was used for detecting H.pylori, and immunohistochemical studies, seminested PCR amplification were carried out to detect clonality in 26 cases of lymphocytic gastritis and 11 cases of controls. The results showed that B cell monoclonality was detected by PCR assay in 26.9% of patients with lymphocytic gastritis, and the detecting rate of monoclonality in lymphocytic gastritis with H.pylori infection was significantly higher than that in lymphocytic gastritis without H.pylori infection ( P <0.05); H.pylori infection was found in 53.8% of the lymphocytic gastritis cases and in 45.4% of the controls( P >0.05). The results suggest that B cell monoclonality, which may be a potential marker of pre neoplastic lesions, is present in some cases of lymphocytic gastritis and may be associated with H.pylori infection, but the latter may not be the only pathogen involoved in B cell monoclonality.