妇科止血消痛颗粒对产后子宫复旧不全大鼠模型子宫MMP-9、TIMP-1mRNA表达的影响

目的观察妇科止血消痛颗粒对产后子宫复旧不全大鼠子宫组织基质金属蛋白-9（MMP-9）、组织金属蛋白酶抑制物-1（TIMP-1）表达的影响，探讨妇科止血消痛颗粒治疗产后子宫复旧不全的机制。方法建立大鼠产后子宫复旧不全病理模型，随机分为模型组、产妇康组及妇科止血消痛颗粒高、中、低剂量组。造模第1日开始各给药组分别灌胃相应药物，空白组、模型组予蒸馏水灌胃。均每日1次，连续7d。采用原位杂交法检测各组大鼠子宫组织中MMP-9、TIMP-1基因的表达水平。结果各组大鼠子宫内膜中均可检测到MMP-9和TIMP-1的杂交信号。模型组MMP-9表达强度明显超过空白组，TIMP-1表达弱于空白组。妇科止血消痛颗粒高、中、低剂量组及产妇康组，MMP-9 mRNA的表达明显降低（P〈0．05，P〈0．01）；妇科止血消痛颗粒中、低剂量组与产妇康组MMP-9 mRNA的表达强度相似，但均低于妇科止血消痛颗粒高剂量组的表达强度（P〈0．01）。妇科止血消痛颗粒高剂量组及产妇康组TIMP-1 mRNA的表达明显升高（P〈0．05），且表达相似。结论妇科止血消痛颗粒通过抑制MMP-9的高表达，升高TIMP-1的低表达，从而发挥治疗产后子宫复旧不全的作用。

Effect of Fuke Zhixuexiaotong Keli on the Expression of MMP-9 and TIMP-1mRNA in Rats Model of Incomplete Puerperal Involution of Uterus

Objective To explore the mechanism of Fuke Zhixuexiaotong Keli in rats model of incomplete puerperal involution of uterus by detecting the expression of metalloproteinases-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in the uterine redintegration incomplete model. Methods Seventy- two SD female rats were randomly divided into 6 groups： blank group, model group, Chanfukang group, and high-, middle-, low-dose of Fuke Zhixuexiaotong Keli group. The model of incomplete puerperal involution of uterus was established, and MMP-9, TIMP-1 gene expression level in each group were detected by in situ hybridization detection of uterine tissue. Results The expression of MMP-9 and TIMP-1 could be successfully detected in each group. The expression of MMP-9 in model group was significantly higher than the control group, and the expression of TIMP-1 was lower. The mRNA of MMP-9 gene was significantly decreased in both high-, middle- and low-dose of Fuke Zhixuexiaotong Keli group and Chanfukang group （P〈0.05, P〈0.01）, the expression of MMP-9 was similar in low-dose of Fuke Zhixuexiaotong Keli group and Chanfukang group, and lower than the high-dose group （P 〈0.01）. TIMP-1 mRNA was significantly higher in high-dose of Fuke Zhixuexiaotong Keli group and Chanfukang group than the other groups, the expression of the two groups was similar （P 〈 0.05）. Conclusion Fuke Zhixuexiaotong Keli can play a role in the treatment of postpartum uterine redintegration incomplete by inhibiting MMP-9 in high expression, promoting TIMP- 1 in low expression.